外源ATP对油菜幼苗耐寒性的影响

doi:10.7525/j.issn.1673-5102.2021.02.018

摘要/Abstract

摘要：

以“陇油7号” 油菜为实验材料，研究了外源ATP对油菜幼苗耐寒性的影响。结果表明：与单独低温胁迫相比，外源ATP预处理再进行低温胁迫后，油菜幼苗MDA含量、含量均显著降低，而叶绿素含量、抗氧化酶活性（SOD、POD、CAT、APX）和RBOHD、RBOHF、CPK4、CPK5基因表达均增加；与外源ATP+低温相比，EGTA+外源ATP+低温处理下，MDA含量显著增加，总叶绿素含量、T-AOC酶活性、RBOHD、RBOHF基因表达均显著下降，DMTU+外源ATP+低温处理下，MDA含量显著增加，总叶绿素含量、Ca²⁺-ATPase酶活性、CPK4、CPK5基因表达均下降，表明外源ATP通过Ca²⁺和H₂O₂依赖性机制影响油菜幼苗的耐寒性。

关键词: 油菜, 外源ATP, 低温胁迫, 钙, 抗氧化酶

Abstract:

The effects of exogenous ATP on the cold tolerance of rape seedlings were studied with “Longyou 7”Brassica campestris L. as experimental material. Compared with low temperature stress alone， MDA content and content of rapeseed seedlings were significantly decreased after exogenous ATP pretreatment and low temperature stress， while chlorophyll content and antioxidant enzyme activities（SOD， POD， CAT， APX） were observed. The expressions of RBOHD， RBOHF， CPK4 and CPK5 were increased. Compared with exogenous ATP+ low temperature， MDA content was significantly increased under EGTA+exogenous ATP+ low temperature treatment. Total chlorophyll content， T-AOC enzyme activity， RBOHD and RBOHF gene expression were all increased. Significantly decreased， MDA content was increased significantly under DMTU+exogenous ATP+low temperature treatment， total chlorophyll content， Ca²⁺-ATPase activity， CPK4， CPK5 gene expression were decreased， indicating that exogenous ATP affected cold tolerance of rape seedlings by Ca²⁺and H₂O₂-dependent mechanisms.

Key words: Brassica campestris L., exogenous ATP, low temperature stress, calcium, antioxidant enzymes

中图分类号:

S565.4

胡馨丹, 李瑶, 张小花, 梁娟红, 张腾国. 外源ATP对油菜幼苗耐寒性的影响[J]. 植物研究, 2021, 41(2): 302-311.

Xin-Dan HU, Yao LI, Xiao-Hua ZHANG, Juan-Hong LIANG, Teng-Guo ZHANG. Effect of Exogenous ATP on Cold Tolerance of Brassica campestris Seedlings[J]. Bulletin of Botanical Research, 2021, 41(2): 302-311.

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基因 Gene	基因号 Genbank number	引物 Primer	碱基序列 Sequence（5’to 3’）
Actin	XM_013830703.2	Actin-F	TGTGCCAATCTACGAGGGTTT
Actin	XM_013830703.2	Actin-R	TTTCCCGCTCTGCTGTTGT
BrRbohD	XM_009153345.2	RbohD-F	CACTAGCTCGCCGTAATAACATCTC
BrRbohD	XM_009153345.2	RbohD-R	CGTCCATCTTCGTCTTTGTCC
BrRbohF	XM_009114548.2	RbohF-F	CAGAGCCGATGAAACAACAAAG
BrRbohF	XM_009114548.2	RbohF-R	GCCAAGACCAACCAATAAGAGAA
BrCPK4	XM_009135901.2	CPK4-F	CAATTCGGGACGACCTATCT
BrCPK4	XM_009135901.2	CPK4-R	GGTGCATGATCTGAATCTCG
BrCPK5	XM_018653206.1	CPK5-F	ACTTTGATTCGGATCCTTGG
BrCPK5	XM_018653206.1	CPK5-R	ACACCGTTCTCACAGATCCA

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