遮荫条件下黄檗生长和生理响应的性别差异研究

doi:10.7525/j.issn.1673-5102.2020.05.012

摘要/Abstract

摘要： 分析遮荫条件下黄檗幼苗形态指标、生理指标和代谢物质性别间差异，探讨其对遮荫的响应策略，为黄檗野生资源保护及造林抚育提供理论依据。以2年生黄檗雌雄株幼苗为试验材料，采用控制实验，设置3个遮荫梯度，自然光为对照，对比研究遮荫条件下黄檗幼苗雌雄植株形态特征、生物量分配、抗氧化防御酶活性、代谢物质的差异，以揭示其遮荫条件下的生存策略。结果表明：遮荫处理更有利于黄檗的生长，轻度遮荫雌株生物量积累大于雄株，重度遮荫后雄株大于雌株；遮荫处理对叶绿素a、总叶绿素含量均有显著影响（P<0.01），性别间差异不显著，遮荫条件有利于促进黄檗幼苗叶绿素积累；遮荫处理对酶活性和MDA含量均有显著影响，性别间差异不显著，SOD酶活性均随遮荫强度的增强而增大，POD和CAT酶活性随遮荫强度的增强而减小，MDA含量在对照处理时最高；遮荫条件下可溶性糖和可溶性淀粉含量下降，性别间差异不显著。黄檗幼苗通过改变构件性状、生物量、抗氧化酶活性及代谢物质含量，适应遮荫环境。研究遮荫对黄檗生长和生理响应的性别差异，以期为遮荫环境下黄檗个体发育、种群形成、适应机制及种群繁衍等研究奠定基础，为雌雄异株植物资源的保护利用提供科学借鉴。

关键词: 黄檗, 遮荫, 形态, 生理, 性别

Abstract: This paper analyzed the differences of morphological indexes, physiological indexes and metabolism indexes caused by the seedlings gender in condition of overshadow, and discussed the strategies response to overshadow, provided theoretical basis for the protection of wild resources and forestation of the seedlings. The differences of morphological characteristics, biomass distribution, antioxidative enzyme activity and metabolism of the biennial Phellodendron amurense seedlings were studied by using the control experiment with three shading gradients and sun light as the control. The results showed that overshadow was more beneficial to the growth of P.amurense, the biomass accumulation of female plants in light shading were better than male plants, but in severe overshadow male plants were better than female plants, overshadow had a significant effects on chlorophyll a and the total chlorophyll content(P<0.01), and there was no significant effect on gender, and shading treatment was promotive to the chlorophyll accumulation of P.amurense seedlings; shading treatment had a significant effect on enzyme activity and MDA content, however, activities of POD and CAT were decreased with the shade intensity increased, MDA content was the highest in the control treatment, soluble sugar and starch content decreased under shading condition, but there was no significant effect on gender. The seedlings of P.amurense adapted to the shade by changing the component properties, biomass, antioxidant enzyme activity and metabolic substance content. The gender differences of the effects of shading on the growth and physiology of P.amurense would lay a foundation for the individual development, population formation, adaptation mechanism and population reproduction of P.amurense under shading environment, and would provide scientific reference for the protection and utilization of dioecious plant resources.

Key words: Phellodendron amurense Rupr., shading, morphology, physiology, gender

中图分类号:

S792.31

张玲, 张东来. 遮荫条件下黄檗生长和生理响应的性别差异研究[J]. 植物研究, 2020, 40(5): 735-742.

ZHANG Ling, ZHANG Dong-Lai. Gender Differences in Growth and Physiological Respond of Phellodendron amurense Rupr. in Condition of Overshadow[J]. Bulletin of Botanical Research, 2020, 40(5): 735-742.

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