白桦MYB基因响应激素及盐旱处理的表达研究

doi:10.7525/j.issn.1673-5102.2020.05.013

摘要/Abstract

摘要： 转录因子参与到植物激素不同的信号通路中及非生物胁迫的调控中，为研究白桦MYB转录因子家族基因对不同激素信号及非生物胁迫的响应，本研究以野生型白桦（Betula platyphylla）种子为材料，分别用10 μmol·L^-1的茉莉酸甲酯（MeJA）、100 μmol·L^-1的乙烯利（ETH）、20 μmol·L^-1的脱落酸（ABA）、50 μmol·L^-1的细胞激动素（KT）、200 mmol·L^-1 NaCl、100 mmol·L^-1 Mannitol以及水为对照进行处理，对激素处理4周的白桦下胚轴进行表型观察及基因表达分析，结果显示：激素处理能够影响白桦下胚轴及胚根的生长，经过ETH处理的白桦下胚轴和根短于水处理的对照，KT处理过的白桦下胚轴和根长于水处理的对照；MeJA、Ethylene及ABA处理对大部分BpMYBs的表达存在负调控，而BpMYB2，8，12基因上调表达；KT处理后，只有BpMYB11下调表达，其他的BpMYBs基因上调表达；NaCl处理后，大部分基因表现出先下调后上调的表达模式，在48 h被高度诱导；Mannitol模拟干旱处理条件下，大部分基因表现出先微弱上调后下调的表达模式。这些结果说明白桦MYB家族基因能够响应激素、盐和旱的处理，在调控白桦下胚轴及胚根应答外界信号的发育中起作用。本研究为探索激素调控木本植物生长发育的分子机制研究提供了数据和材料。

关键词: 白桦, BpMYBs, 胁迫处理, 表达模式

Abstract: Transcription factors are involved in different signaling pathways of plant hormones and the regulation of abiotic stress. In order to study the response of birch MYB transcription factor family genes to different hormone signals and abiotic stress, we used wild-type birch(Betula platyphylla) seeds:10 μmol·L^-1 methyl jasmonate(MeJA), 100 μmol·L^-1 ethylene glycol(ETH), 20 μmol·L^-1 abscisic acid(ABA), 50 μmol·L^-1 cytokinin(KT), 200 mmol·L^-1 NaCl, 100 mmol·L^-1 Mannitol and water were used as controls. Phenotypic observation and gene expression analysis were performed on hormone-treated 4 weaks birch hypocotyls. The hormone treatment could affect the growth of birch hypocotyls and radicles. The hypocotyls and roots of the treated birch were shorter than those of the water-treated control, and the KT-treated birch hypocotyls and roots were longer than those of the water-treated control; MeJA, Ethylene, and ABA treatment negatively regulated the expression of most BpMYBs, while BpMYB2,8,12 genes were up-regulated. After KT treatment, only BpMYB11 was down-regulated, and other BpMYBs genes were up-regulated. After NaCl treatment, most genes showed an expression pattern of down-regulation and then up-regulation, and was highly induced at 48 h; Mannitol simulated under drought conditions, most of the gene expression pattern showed slight increase after the first cut. These results indicate that birch MYB family genes can respond to hormone, salt and drought treatments, and play a role in regulating the development of birch hypocotyls and radicles in response to external signals. This study provides data and materials for exploring the molecular mechanism of hormone-regulated woody plant growth and development.

Key words: Betula platyphylla, BpMYB genes, stress treatment, expression pattern

中图分类号:

S792.153

刘佳欣, 刘慧子, 石晶静, 于颖, 王超. 白桦MYB基因响应激素及盐旱处理的表达研究[J]. 植物研究, 2020, 40(5): 743-750.

LIU Jia-Xin, LIU Hui-Zi, SHI Jing-Jing, YU Ying, WANG Chao. Expression of MYB Genes of Birch in Response to Hormones,Salt and Drought[J]. Bulletin of Botanical Research, 2020, 40(5): 743-750.

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