低温胁迫下结球甘蓝BoNR8 lncRNA过表达对拟南芥种子萌发的影响

doi:10.7525/j.issn.1673-5102.2020.03.016

植物研究 ›› 2020, Vol. 40 ›› Issue (3): 441-446.doi: 10.7525/j.issn.1673-5102.2020.03.016

低温胁迫下结球甘蓝BoNR8 lncRNA过表达对拟南芥种子萌发的影响

杨贺¹, 张楠¹, 刘自广², 林建辉¹, 孙世臣³, 刘圣怡⁴, 岑曦¹, 吴娟¹

1. 东北盐碱植被恢复与重建教育部重点实验室, 生命科学学院, 东北林业大学, 哈尔滨 150040;
2. 黑龙江省农业科学院畜牧研究所, 哈尔滨 150028;
3. 黑龙江省农业科学院耕作栽培研究所, 哈尔滨 150028;
4. 牡丹江医学院, 牡丹江 157011

收稿日期:2019-10-17 出版日期:2020-05-05 发布日期:2020-05-29
通讯作者: 吴娟,E-mail:wuj1970@163.com E-mail:wuj1970@163.com
作者简介:杨贺(1995-),女,硕士研究生,主要从事生物化学与分子生物学方面的研究。
基金资助:
黑龙江省科学基金项目（LH2019C007）；中央高校基本科研业务费专项资金资助（2572019CT03）；东北油田盐碱植被恢复与重建教育部重点实验室开放基金（SAVER1702）

Expression and Function of BoNR8 lncRNA in Cabbage under Cold Stress

YANG He¹, ZHANG Nan¹, LIU Zi-Guang², LIN Jian-Hui¹, SUN Shi-Chen³, LIU Sheng-Yi⁴, CEN Xi¹, WU Juan¹

1. Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field of Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040;
2. Institute of Animal Science, Heilongjiang Academy of Agricultural Sciences, Harbin 150028;
3. Institute of Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, Harbin 150028;
4. Mudanjiang Medical University, Mudanjiang 157011

Received:2019-10-17 Online:2020-05-05 Published:2020-05-29
Supported by:
Heilongjiang Provincial Science Fund Project(LH2019C007);Fundamental Research Funds for Central Universities(2572019CT03);Key Laboratory of Salt and Alkali Vegetation Restoration and Reconstruction of the Northeast Oilfield(SAVER1702)

摘要/Abstract

摘要： 长非编码RNA（long no-coding RNA，lncRNA）不含有开放阅读框，且长度大于200 bp，一般不具有编码蛋白质功能。它们在生物体内普遍存在，并发挥着多种生物学作用。植物种子萌发期的特异表达的lncRNA研究较少。BoNR8 lncRNA是结球甘蓝中RNA聚合酶Ⅲ转录的长非编码RNA（约272 bp）。前期研究发现，它在种子萌发期的特异表达；拟南芥中BoNR8 lncRNA过表达抑制了正常条件下种子萌发并降低了萌发种子对ABA的敏感性。本研究对BoNR8 lncRNA序列分析发现，其转录区域内存在冷胁迫基序（ATATAAATAAAT），并且这个基序存在BoNR8 lncRNA二级结构的最大茎环中。生化实验进一步表明，BoNR8 lncRNA响应低温环境，其过表达抑制了低温胁迫下拟南芥种子萌发。BoNR8 lncRNA低温相关功能的发现为植物耐低温研究提供了新材料，丰富了植物耐低温作用机制的研究。

关键词: 长非编码RNA, 结球甘蓝, 冷应激, 种子萌发

Abstract: Long non-coding RNA(lncRNA) with longer than 200 bp in length do not contain open reading frames, and they generally do not have a protein-coding function. They are ubiquitous in the body and play a variety of biological roles. There are few studies on lncRNA specifically expressed in the germination stage of plant seeds. BoNR8 lncRNA is a long non-coding RNA(about 272 bp) transcribed by RNA polymerase Ⅲ in cabbage. Previous studies have found that it is specifically expressed in the seed germination stage, and the overexpression of BoNR8 lncRNA in Arabidopsis inhibits seed germination under normal conditions and reduces the sensitivity of germinated seeds to ABA. In this study, we analyzed the sequence of BoNR8 lncRNA and found that there was a cold stress motif(ATATAAATAAAT) in the transcribed region, and this motif exists in the largest stem loop of the secondary structure of BoNR8 lncRNA. Biochemical experiments further showed that BoNR8 lncRNA responded to low temperature environment, and its overexpression inhibited seed germination of Arabidopsis thaliana under low temperature stress. The discovery of low-temperature related functions of BoNR8 lncRNA provides new materials for low temperature resistance research of plant and enriches the study of low temperature resistance mechanism of plant.

Key words: long non coding RNA, Brassica oleracea L., cold stress, seed germination

中图分类号:

S635.1

杨贺, 张楠, 刘自广, 林建辉, 孙世臣, 刘圣怡, 岑曦, 吴娟. 低温胁迫下结球甘蓝BoNR8 lncRNA过表达对拟南芥种子萌发的影响[J]. 植物研究, 2020, 40(3): 441-446.

YANG He, ZHANG Nan, LIU Zi-Guang, LIN Jian-Hui, SUN Shi-Chen, LIU Sheng-Yi, CEN Xi, WU Juan. Expression and Function of BoNR8 lncRNA in Cabbage under Cold Stress[J]. Bulletin of Botanical Research, 2020, 40(3): 441-446.

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低温胁迫下结球甘蓝BoNR8 lncRNA过表达对拟南芥种子萌发的影响

Expression and Function of BoNR8 lncRNA in Cabbage under Cold Stress

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