松栎柱锈菌春孢子多糖体外抗氧化活性

doi:10.7525/j.issn.1673-5102.2017.04.020

植物研究 ›› 2017, Vol. 37 ›› Issue (4): 628-634.doi: 10.7525/j.issn.1673-5102.2017.04.020

松栎柱锈菌春孢子多糖体外抗氧化活性

邸雪颖, 贾舒涵, 薛煜, 杨光, 赵博, 瓮岳太

东北林业大学林学院, 哈尔滨 150040

收稿日期:2017-01-17 出版日期:2017-07-15 发布日期:2017-07-22
通讯作者: 瓮岳太,E-mail:1150599114@qq.com E-mail:1150599114@qq.com
作者简介:邸雪颖(1957-),男,教授,主要从事森林防火方面的研究。
基金资助:
国家林业局"948"项目（2015-4-35）

Antioxidant Activities of Polysaccharides from Cronartium orientale Aeciospores in vitro

DI Xue-Ying, JIA Shu-Han, XUE Yu, YANG Guang, ZHAO Bo, WENG Yue-Tai

School of forestry, Northeast Forestry University, Harbin 150040

Received:2017-01-17 Online:2017-07-15 Published:2017-07-22
Supported by:
State Bureau of Forestry 948 project(2015-4-35)

摘要/Abstract

摘要： 为了研究松栎柱锈菌（Cronartium orientale S.Kaneko）春孢子多糖的体外抗氧化活性，制备了该菌破壁春孢子精制多糖，并对所得春孢子多糖进行了总抗氧化能力、总还原力以及清除二苯代苦味酰基（DPPH·）、清除羟基自由基（·OH）、清除超氧阴离子（O₂^·）的活性进行了测定。体外抗氧化活性分析结果表明：松栎柱锈菌春孢子多糖具有较强的总抗氧化能力；虽然该菌多糖不具有清除多数氧自由基母体（O₂^·）的作用，也不具有清除人工合成自由基DPPH·的作用，但是其能够有效清除对机体破坏力最强的自由基·OH。这表明本文获得的松栎柱锈菌春孢子多糖具有对自由基选择性清除的能力，对其他自由基研究者具有参考价值，可为该菌的开发利用提供参考，也能够为森林有害生物开发利用提供理论依据。

关键词: 松栎柱锈菌春孢子, 多糖, 自由基, 抗氧化

Abstract: We prepared the wall-breaking Cronartium orientale aeciospore refined polysaccharides to study the antioxidant activities in vitro, and assayedthe polysaccharides' bioactivities including total antioxidant capacity, total reducing power, and capacities of scavenging 1,1-diphenyl-2-picrylhydrazyl free radicals(DPPH·), scavenging hydroxyl free radical(·OH), scavenging superoxide anion(O₂^·). The polysaccharides' reducing power was lower than Vc, but with a strong total antioxidant capacity.The matrix of most of oxygen free radicals O₂^· and artificial free radical DPPH· could not be scavenged,but it could effectively scavenge the organism's most destructive free radical ·OH. C.orientale aeciospore polysaccharides could scavenge free radicals selectively. Therefore, the polysaccharides possessed potential value on exploitation and could provide reference to other researchers and a theoretical basis for exploitation of forest pests.

Key words: Cronartium orientale aeciospores, polysacchrides, free radicals, antioxidation

中图分类号:

S432

邸雪颖, 贾舒涵, 薛煜, 杨光, 赵博, 瓮岳太. 松栎柱锈菌春孢子多糖体外抗氧化活性[J]. 植物研究, 2017, 37(4): 628-634.

DI Xue-Ying, JIA Shu-Han, XUE Yu, YANG Guang, ZHAO Bo, WENG Yue-Tai. Antioxidant Activities of Polysaccharides from Cronartium orientale Aeciospores in vitro[J]. Bulletin of Botanical Research, 2017, 37(4): 628-634.

参考文献

1. 薛煜,邵力平,马泽芳,等. 兴凯湖松瘤锈病病、健组织中主要有机成分变化及其对结实的影响[J]. 东北林业大学学报,1992,20(2):30-34.Xue Y,Shao L P,Ma Z F,et al. Change in main organic matter contents between infected and healthy tissues and the effect of Xinkai Lake pine rust to fruiting ability[J]. Journal of Northeast Forestry University,1992,20(2):30-34.
2. 邵力平,薛煜,王振华,等. 兴凯湖松瘤锈病损失量的研究[J]. 东北林业大学学报,1989,17(2):1-5.Shao L P,Xue Y,Wang Z H,et al. Study on the losses of Xingkai Lake pine gall rust[J]. Journal of Northeast Forestry University,1989,17(2):1-5.
3. 翁梁,温鲁. 药用真菌多糖研究进展[J]. 食品科学,2008,29(12):748-751.Weng L,Wen L. Review of research on medicinal fungi polysaccharides[J]. Food Science,2008,29(12):748-751.
4. San-blas G,Suzuki S,Hearn V,et al. Fungal polysaccharides[J]. Journal of Medical and Veterinary Mycology,1994,32(S1):321-328.
5. Lee S K,Kakishima M. Aeciospore surface structures of Gymnosporangium and Roestelia(Uredinales)[J]. Mycoscience,1999,40(2):109-120.
6. Wahyuno D,Kakishima M,Ono Y. Aeciospore-surface structures of Phragmidium species parasitic on roses[J]. Mycoscience,2002,43(2):159-167.
7. Meng S,Ma Y,Sun D W,et al. Properties of starch-palmitic acid complexes prepared by high pressure homogenization[J]. Journal of Cereal Science,2014,59(1):25-32.
8. Oboroceanu D,Wang L Z,Kroes-nijboer A,et al. The effect of high pressure microfluidization on the structure and length distribution of whey protein fibrils[J]. International Dairy Journal,2011,21(10):823-830.
9. 范本隽,张志森. 高压均质机超细粉碎机理探讨[J]. 华东理工大学学报,2004,30(1):96-98.Fan B J,Zhang Z S. Super-fine mechanism of the high-pressure homogenizer[J]. Journal of East China University of Science and Technology,2004,30(1):96-98.
10. 刘晓涵,陈永刚,林励,等. 蒽酮硫酸法与苯酚硫酸法测定枸杞子中多糖含量的比较[J]. 食品科技,2009,34(9):270-272.Liu X H,Chen Y G,Lin L,et al. Comparison of methods in determination of polysaccharide in Lycium barbarum L. [J]. Food Science and Technology,2009,34(9):270-272.
11. Lin C L,Wang C C,Chang S C,et al. Antioxidative activity of polysaccharide fractions isolated from Lycium barbarum Linnaeus. [J]. International Journal of Biological Macromolecules,2009,45(2):146-151.
12. Oyaizu M. Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine[J]. The Japanese Journal of Nutritionand Dietetics,1986,44(6):307-315.
13. 戴晓红. DNA分子中碱基甲基化及羟基化损伤的理论研究[D]. 南宁:广西师范大学,2008.Dai X H. Theoretical studies on the base damage of methylation and hydroxylation in DNA[D]. Nanning:Guangxi Normal University,2008.
14. 林菁. NO对羟基(·OH)损伤DNA的保护作用[D]. 广州:广州中医药大学,2014.Lin J. Protective effect and mechanism of nitric oxide against hydroxyl radical-induced DNA damage[D]. Guangzhou:Guangzhou University of Chinese Medicine,2014.
15. 黄斌,王少华. 一氧化氮及其供体在儿科的临床应用新进展[J]. 医学综述,2012,18(14):2175-2177.Huang B,Wang S H. Recent progress of nitric oxide and its donor in the clinical application of pediatrics[J]. Medical Recapitulate,2012,18(14):2175-2177.
16. 刁玲. 羟基自由基和核酸碱基对反应机理的理论研究[D]. 上海:上海大学,2015.Diao L. Theoretical research the reaction of OH radical with base pair in nucleic acid[D]. Shanghai:Shanghai University,2015.
17. Fu L,Xu B T,Xu X R,et al. Antioxidant capacities and total phenolic contents of 56 wild fruits from South China[J]. Molecules,2010,15(12):8602-8617.
18. 汤俊,胡征宇. 3种念珠藻多糖对自由基的清除作用[J]. 武汉植物学研究,2006,24(1):63-66.Tang J,Hu Z Y. Radical scavenging activities of polysaccharides in three Nostoc species[J]. Journal of Wuhan Botanical Research,2006,24(1):63-66.
19. 徐静静. 竹荪子实体水不溶性多糖的化学修饰及活性研究[D]. 无锡:江南大学,2012.Xu J J. Studies on chemical modification and bioactivities of water-insoluble polysacchairdes from Dictyophora indusiata[D]. Wuxi:Jiangnan University,2012.
20. Moskovitz J,Yim M B,Chock P B. Free radicals and disease[J]. Archives of Biochemistry and Biophysics,2002,397(2):354-359.
21. 王崔平. 活性氧自由基的检测及其清除研究[D]. 北京:首都师范大学,2007.Wang C P. Detection and scavenging of reactive oxygen species[D]. Beijing:Capital Normal University,2007.
22. Feyzi E,Sundheim O,Westbye M P,et al. RNA base damage and repair[J]. Current Pharmaceutical Biotechnology,2007,8(6):326-331.
23. Huang S Q,Ding S D,Fan L P. Antioxidant activities of five polysaccharides from Inonotus obliquus[J]. International Journal of Biological Macromolecules,2012,50(5):1183-1187.

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松栎柱锈菌春孢子多糖体外抗氧化活性

Antioxidant Activities of Polysaccharides from Cronartium orientale Aeciospores in vitro

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