土壤铜对凤丹籽油含量和成分的影响

doi:10.7525/j.issn.1673-5102.2017.01.020

摘要/Abstract

摘要： 高含量不饱和脂肪酸，特别是高含量亚麻酸是牡丹籽油品质的主要体现，但到目前为止，在凤丹传统栽培区（铜矿区）土壤铜含量是否影响牡丹籽油品质并没有被调查。本研究通过调查安徽省凤凰山-丫山30个凤丹（Paeonia ostii）栽培区土壤Cu元素含量和凤丹籽油组成，显示凤丹栽培区土壤铜含量为18.98~298.82 mg·kg^-1，变异系数为83.06%；凤丹籽油中棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸5种主要脂肪酸含量分别为5.62%、1.89%、24.59%、29.76%、38.13%，变异系数在5.66~9.72，其中亚油酸变异系数最高为9.72；土壤和叶片中Cu含量与亚油酸和不饱和脂肪酸含量均存在明显的负相关性，与亚麻酸含量没有显著相关性；土壤和叶片中Cu含量呈显著正相关，r=0.778。以上表明以油用为目的的凤丹栽培应该避免土壤中铜含量过高影响牡丹籽油品质。

关键词: 凤丹, 籽油, 脂肪酸, 不饱和脂肪酸, Cu污染

Abstract: The unsaturated fatty acids, especially linolenic acid are the main embodiment of the quality of peony seed oil. But so far, in traditional cultivation area of Paeonia ostii, it has not been investigated whether the soil copper content affected the quality of peony seed oil. In this study, we investigated copper content in soil and the composition of the seed oil in 30 P.ostii cultivation areas in Anhui Province. The copper content in the soil was 18.98-298.82 mg·kg^-1, and the variation coefficient was 83.06%. The contents of palm acid, stearic acid, oleic acid, linoleic acid and linoleic acid were 5.62%, 1.89%, 24.59%, 29.76% and 38.13%, respectively. The variation coefficient was between 5.66 and 9.72, among which the highest one was linoleic acid. There was a significantly negative correlation between copper content and unsaturated fatty acid content, linoleic acid content, and there was no significant correlation between the content of copper and linolenic acid content. And there was a significantly positive correlation between soil copper content and leaf copper content, r=0.778. The cultivation of P.ostii for oil production should avoid the high copper content in the soil to keep the quality of peony seed oil.

Key words: Paeonia ostii, seed oil, fatty acid, unsaturated fatty acids, copper pollution

中图分类号:

赵晓菊, 秦薇, 陈华峰. 土壤铜对凤丹籽油含量和成分的影响[J]. 植物研究, 2017, 37(1): 155-160.

ZHAO Xiao-Ju, QIN Wei, CHEN Hua-Feng. Effect of Cu on Compositions of Seed Oil in Paeonia ostii[J]. Bulletin of Botanical Research, 2017, 37(1): 155-160.

参考文献

1. 洪德元,潘开玉. 芍药属牡丹组的分类历史和分类处理[J]. 植物分类学报,1999,37(4):351-368.Hong D Y,Pan K Y. Taxonomical history and revision of Paeonia sect. Moutan(Paeoniaceae)[J]. Acta Phytotaxonomica Sinica,1999,37(4):351-368.
2. 李晓青,韩继刚,刘炤,等. 不同地区凤丹经济性状及其籽油脂肪酸成分分析[J]. 粮食与油脂,2014,27(4):43-46.Li X Q,Han J G,Liu Z,et al. Economic characteristics investigation and seed oil fatty acid composition analysis of Paeonia ostii plants in different areas[J]. Cereals & Oils,2014,27(4):43-46.
3. 韩继刚,李晓青,刘炤,等. 牡丹油用价值及其应用前景[J]. 粮油与油脂,2014,27(5):21-25.Han J G,Li X Q,Liu Z,et al. Potential applications of tree peony as an oil plant[J]. Cereals & Oils,2014,27(5):21-25.
4. Zhang Z H,Wang H,Liu J,et al. The effects of soil metals on the composition of oil of Paeonia ostii seeds[J]. Journal of Plant Interactions,2015,10(1):288-295.
5. 刘登义,沈章军,严密,等. 铜陵铜矿区凤丹根际和非根际土壤酶活性[J]. 应用生态学报,2006,17(7):1315-1320.Liu D Y,Shen Z J,Yan M,et al. Enzyme activities in Paeonia ostii rhizosphere and non-rhizosphere soil of Tongling copper mining[J]. Chinese Journal of Applied Ecology,2006,17(7):1315-1320.
6. Xu D C,Zhou P,Zhan J,et al. Assessment of trace metal bioavailability in garden soils and health risks via consumption of vegetables in the vicinity of Tongling mining area,China[J]. Ecotoxicology and Environmental Safety,2013,90:103-111.
7. 王少华,杨劼,刘苏明,等. 铜陵狮子山杨山冲尾矿库重金属元素释放的环境效应[J]. 高校地质学报,2011,17(1):93-100.Wang S H,Yang J,Liu S M,et al. Environmental effects of heavy metal elements release in Yangshanchong tailing pool,Shizishan,Tongling,Anhui province[J]. Geological Journal of China Universities,2011,17(1):93-100.
8. 沈章军,王友保,王广林,等. 铜陵铜尾矿凤丹种植基地重金属污染初探[J]. 应用生态学报,2005,16(4):673-677.Shen Z J,Wang Y B,Wang G L,et al. Heavy metals pollution of Paeonia ostii land at copper-tailings reservoir of Tongling city:a preliminary study[J]. Chinese Journal of Applied Ecology,2005,16(4):673-677.
9. 陈怀满. 土壤中化学物质的行为与环境质量[M]. 北京:科学出版社,2002.Chen H M. Behavior of chemicals in soils and its relation to environmental quality[M]. Beijing:Science Press,2002.
10. 沈昌高,高超,王登峰,等. 铜陵矿区土壤和油菜中铜的分布特征[J]. 应用生态学报,2007,18(10):2374-2378.Shen C G,Gao C,Wang D F,et al. Distribution characteristics of copper in soil and rape around Tongling mining area[J]. Chinese Journal of Applied Ecology,2007,18(10):2374-2378.
11. Phippen W B,Isbell T A,Phippen M E. Total seed oil and fatty acid methyl ester contents of Cuphea accessions[J]. Industrial Crops and Products,2006,24(1):52-59.
12. Adriano D C. Trace elements in terrestrial environments:biogeochemistry,bioavailability and risks of metals[M]. 2nd ed. New York:Springer-Verlag,2001.
13. 韩雪源,张延龙,牛立新,等. 不同产地‘凤丹’牡丹籽油主要脂肪酸成分分析[J]. 食品科学,2014,35(22):181-184.Han X Y,Zhang Y L,Niu L X,et al. Fatty acid composition of ‘Fengdan’ peony seed oils from different growing regions[J]. Food Science,2014,35(22):181-184.
14. 史国安,郭香凤,金宝磊,等. 牡丹籽油超临界CO₂萃取工艺优化及抗氧化活性的研究[J]. 中国粮油学报,2013,28(4):47-50,107.Shi G A,Guo X F,Jin B L,et al. Optimization of supercritical CO₂ extraction and analysis of antioxidation activity of peony seed oil[J]. Journal of the Chinese Cereals and Oils Association,2013,28(4):47-50,107.
15. 高婷婷,王亚芸,任建武. GC-MS法分析牡丹籽油的成分及其防晒效果的评定[J]. 食品科技,2013,38(6):296-299.Gao T T,Wang Y Y,Ren J W. Analysis of the composition in peony seed oil by GC/MS and evaluation in the sunscreen effect[J]. Food Science and Technology,2013,38(6):296-299.
16. 周海梅,马锦琦,苗春雨,等. 牡丹籽油的理化指标和脂肪酸成分分析[J]. 中国油脂,2009,34(7):72-74.Zhou H M,Ma J Q,Miao C Y,et al. Physicochemical indexes and fatty acid composition of peony seed oil[J]. China Oils and Fats,2009,34(7):72-74.
17. 王昌涛,张萍,董银卯. 超临界CO₂提取牡丹籽油的工艺以及成分分析[J]. 中国粮油学报,2009,24(8):96-99,107.Wang C T,Zhang P,Dong Y M. The technique of the extraction of oil from peony seed with supercritical CO₂ extraction method and the analysis of the composition[J]. Journal of the Chinese Cereals and Oils Association,2009,24(8):96-99,107.
18. Khurana N,Singh M V,Chatterjee C. Copper stress alters physiology and deteriorates seed quality of rapeseed[J]. Journal of Plant Nutrition,2006,29(1):93-101.
19. De Vos C H R,Vonk M J,Vooijs R,et al. Glutathione depletion due to copper-induced phytochelatin synthesis causes oxidative stress in Silene cucubalus[J]. Plant Physiology,1992,98(3):853-858.
20. Ohlrogge J,Browse J. Lipid biosynthesis[J]. The Plant Cell,1995,7(7):957-970.
21. Hills M J. Control of storage-product synthesis in seeds[J]. Current Opinion in Plant Biology,2004,7(3):302-308.
22. Sinha S,Sinam G,Mishra R K,et al. Metal accumulation,growth,antioxidants and oil yield of Brassica juncea L. exposed to different metals[J]. Ecotoxicology and Environmental Safety,2010,73(6):1352-1361.
23. Fernandes J C,Henriques F S. Biochemical,physiological,and structural effects of excess copper in plants[J]. The Botanical Review,1991,57(3):246-273.
24. Lidon F C,Henriques F S. Changes in the thylakoid membrane polypeptide patterns triggered by excess Cu in rice[J]. Photosynthetica,1993,28:109-117.
25. Jones G L,Nichols P D,Johns R B,et al. The effect of mercury and cadmium on the fatty acid and sterol composition of the marine diatom Asterionella glacialis[J]. Phytochemistry,1987,26(5):1343-1348.
26. 田生科,李廷轩,杨肖娥,等. 植物对铜的吸收运输及毒害机理研究进展[J]. 土壤通报,2006,37(2):387-394.Tian S K,Li T X,Yang X E,et al. Mechanisms of copper uptake,transportation and detoxification in plants[J]. Chinese Journal of Soil Science,2006,37(2):387-394.
27. Chaffai R,Elhammadi M A,Seybou T N,et al. Altered fatty acid profile of polar lipids in maize seedlings in response to excess copper[J]. Journal of Agronomy and Crop Science,2007,193(3):207-217.
28. Baryla A,Laborde C,Montillet J L,et al. Evaluation of lipid peroxidation as a toxicity bioassay for plants exposed to copper[J]. Environmental Pollution,2000,109(1):131-135.
29. 刘雁丽,史萍,夏妍,等. Cu和丹皮酚磺酸钠处理对凤丹根系生长、丹皮酚含量及H⁺-ATPase活性的影响[J]. 植物资源与环境学报,2012,21(1):20-27.Liu Y L,Shi P,Xia Y,et al. Effects of Cu and sodium paeonol sulfonate treatments on growth,paeonol content and H⁺-ATPase activity in root of Paeonia suffruticosa ‘Feng Dan’ seedling[J]. Journal of Plant Resources and Environment,2012,21(1):20-27.
30. 张敏,江建华,徐洁. 铜矿尾矿库复垦种植牡丹可行性研究[J]. 上海环境科学,2000,19(12):585-587.Zhang M,Jiang J H,Xu J. Feasibility study of reclamation of copper-ore-tailings reservoir for planting peony[J]. Shanghai Environmental Science,2000,19(12):585-587.
31. 周燕,张桂花,徐迎春,等. 铜对凤丹生长、生理及体内矿质元素吸收分配的影响[J]. 生态学杂志,2011,30(3):415-423.Zhou Y,Zhang G H,Xu Y C,et al. Effects of Cu on Paeonia ostii growth,physiology,and element absorption and allocation[J]. Chinese Journal of Ecology,2011,30(3):415-423.
32. 张衷华,唐中华,杨逢建,等. 两种主要油用牡丹光合特性及其微环境影响因子分析[J]. 植物研究,2014,34(6):770-775.Zhang Z H,Tang Z H,Yang F J,et al. Photosynthetic characteristics and its micro-environmental limiting factors of two main oil peony[J]. Bulletin of Botanical Research,2014,34(6):770-775.
33. 邹佳佳,孟梅,张云,等. 农田土壤铜污染评价和油菜铜积累特征研究[J]. 土壤通报,2015,46(3):621-627.Zou J J,Meng M,Zhang Y,et al. Evaluation on copper pollution status of soil and copper accumulation features of rapes in farm land[J]. Chinese Journal of Soil Science,2015,46(3):621-627.

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