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植物研究 ›› 2017, Vol. 37 ›› Issue (5): 690-699.doi: 10.7525/j.issn.1673-5102.2017.05.008

• 研究报告 • 上一篇    下一篇

牡丹胚培养快速成苗技术研究

徐莉, 成仿云, 钟原   

  1. 北京林业大学园林学院国家花卉工程技术中心, 北京 100083
  • 收稿日期:2017-03-27 出版日期:2017-09-15 发布日期:2017-09-15
  • 通讯作者: 成仿云,E-mail:chengfy@263.net E-mail:chengfy@263.net
  • 作者简介:徐莉(1994-),女,硕士研究生,主要从事园林植物与观赏园艺方面的研究。
  • 基金资助:
    北京市支持中央在京高校共建项目(2016GJ-02)

Study on Rapid Seedling-raising Technology of Tree Peony Embryo Culture

XU Li, CHENG Fang-Yun, ZHONG Yuan   

  1. College of Landscape Architecture, Beijing Forestry, National Flower Engineering Technology Research Center, Beijing 100083
  • Received:2017-03-27 Online:2017-09-15 Published:2017-09-15
  • Supported by:
    Beijing supports the common construction project in central University(2016GJ-02)

摘要: 以‘凤丹’及紫斑牡丹种胚为外植体,通过研究激素、光照条件、培养基成分及活性炭(AC)等对离体胚培养的影响,建立了牡丹胚培养直接成苗的高效、快捷的方法。主要结果:在改良MS(钙加倍)+0.2 g·L-1AC+0.5 mg·L-1BA+0.5 mg·L-1GA中离体胚10 d胚根萌动,20 d长出真叶,25 d根系发达,40 d移栽,3个月后幼苗长势良好。实验得出适宜‘凤丹’的培养基:改良MS(钙加倍、大量元素加倍)+0.6 g·L-1AC+1.0 mg·L-1GA,紫斑牡丹:改良MS(钙加倍、大量元素加倍)+0.6 g·L-1AC+0.5 mg·L-1GA;其中‘凤丹’的成苗率63.88%,成活率66.34%,紫斑成苗率93.75%,成活率在品种间有差异,最佳可达100%。

关键词: 胚培养, ‘凤丹’, 紫斑牡丹, 活性炭

Abstract: This study used Paeonia ostii ‘Fengdan’ and Paeonia rockii as explants, established efficient method of Seedling-raising of tree peony embryo culture through the study of hormones, light conditions, medium ingredients and activated carbon(AC) on the in vitro embryo culture. The main result:In vitro embryos radicle germination in 10 d, grow true leaves in 20 d, root system developed in 25 d, transplanting in 40 d, after 3 months the seedlings grow well in the medium of improved MS(calcium doubled)+0.2 g·L-1AC+0.5 mg·L-1GA+0.5 mg·L-1BA. Suitable medium for ‘Fengdan’:improved MS(calcium doubled,macroelement doubled)+0.6 g·L-1AC+1.0 mg·L-1GA; medium for P.rockii:improved MS(calcium doubled,macroelement doubled)+0.6 g·L-1 AC+0.5 mg·L-1GA; The seedling rate of ‘Fengdan’ was 63.88%, the survival rate was 66.34%, and the rate of seedling of P.rockii was 93.75%, and survival rates vary among breeds, with a maximum of 100%.

Key words: embryo culture, Paeonia ostii ‘Fengdan’, Paeonia rockii, activated carbon

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