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植物研究 ›› 2020, Vol. 40 ›› Issue (2): 308-313.doi: 10.7525/j.issn.1673-5102.2020.02.019

• 研究简报 • 上一篇    下一篇

松籽油的干式酶法提取工艺优化与理化性质分析

祖述冲   

  1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040
  • 收稿日期:2019-10-15 出版日期:2020-03-15 发布日期:2020-03-06
  • 作者简介:祖述冲(1983-),男,博士研究生,主要从事森林植物资源中目的活性物质生态分离的研究。

Optimization of Dry Enzymatic Extraction Process and Physicochemical Properties of Pine Seed Oil

ZU Shu-Chong   

  1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2019-10-15 Online:2020-03-15 Published:2020-03-06

摘要: 应用单因素实验设计对干式酶法松籽油提取工艺进行优化。结果表明,干式酶法提取松籽油最佳制备工艺为:0.2%淀粉酶、料液比为6:1、酶解温度为55℃、酶解时间为8 h,所得最大出油率为90.2%,残油率为6.2%。经检测最佳制备条件提取的松籽油不饱和脂肪酸的含量为90.09%,其中油酸含量为26.84%,介酸含量为2.41%、亚油酸含量为46.25%、皮诺敛酸为14.59%;饱和脂肪酸中棕榈酸含量为6.5%、硬脂酸为3.41%。松籽油酸价为2.61 mg·g-1、过氧化值为1.56 mmol·kg-1、丙二醛含量为0.41 mg·kg-1。所有检测结果均高于《中华人民共和国粮食行业标准LS/T 3242-2014》松籽油中质量标准的要求。

关键词: 松籽油, 干式酶法, 单因素实验, 皮诺敛酸, 理化性质

Abstract: Used a single factor experiment to optimize a dry enzyme extraction process of pine seed oil. The best preparation process for dry enzymatic extraction of pine seed oil was:0.2% of amylase, the ratio of material to liquid of 6:1, the hydrolysis temperature of 55℃, and the hydrolysis time of 8 h, and the maximum oil yield 90.2% with the residual oil rate of 6.2%. The content of unsaturated fatty acids in pine seed oil extracted after testing for the best preparation conditions was 90.09%, of which oleic acid content was 26.84%, mediating acid content was 2.41%, linoleic acid content was 46.25%, and pinolacol acid was 14.59%. The content of palmitic acid in saturated fatty acids was 6.5%, and the stearic acid was 3.41%. The pine oil had an acid value of 2.61 mg·g-1, a peroxide value of 1.56 mmol·kg-1, and a malondialdehyde content of 0.41 mg·kg-1. All the test results are higher than the requirements of the quality standards in pine seed oil in the "Food Industry Standard of the People's Republic of China LS/T 3242-2014".

Key words: pine seed oil, dry enzymatic method, single factor experiment, pinolenic acid, physical and chemical properties

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