低温胁迫下外源脱落酸对高山离子芥抗氧化酶和渗透调节物质的影响

doi:10.7525/j.issn.1673-5102.2016.06.010

摘要/Abstract

摘要： 以高山离子芥（Chorispora bungeana）试管苗为实验材料，研究了低温冷害（4℃）、低温冻害（-4℃）和临界温度（0℃）胁迫下外源ABA对抗氧化酶及渗透调节物质的影响。结果表明在4和-4℃胁迫下阴性对照组中SOD和CAT活性高于空白对照组；POD活性在4℃胁迫下高于空白对照组；APX活性在4和-4℃胁迫的晚期升高。与此同时，阴性对照组中膜的相对电导率增加、脯氨酸、可溶性糖含量降低。0℃胁迫下阴性对照组中POD活性降低；APX活性呈波动式变化；脯氨酸和可溶性糖含量分别在72和48 h时高于空白对照组；而可溶性蛋白含量低于空白对照组。添加外源ABA处理后4、-4和0℃胁迫下POD、SOD、CAT和APX活性较阴性对照组均得到不同程度增加；ABA的处理显著降低了细胞膜的相对电导率，提高脯氨酸，可溶性糖和可溶性蛋白的含量。

关键词: 低温胁迫, 高山离子芥, 脱落酸, 抗氧化酶, 渗透调节物

Abstract: Using plantlets of Choraspora bungeana as material, researched the effect of exogenous ABA on antioxidant enzyme and osmotic regulation substances under chilling(4℃), freezing(-4℃) and critical temperature(0℃) were studied. The results showed the activities of SOD and CAT in negative control group were higher than blank control group under 4℃ and -4℃. In negative control group the POD activity was higher than blank control group under 4℃. The APX activity increased at the late period of 4℃ and -4℃ treatment in negative control group. Meanwhile the relative conductivity increased but proline and soluble sugar decreased in negative control group. At 0℃, in negative control group the POD activity decreased and the APX activity changed with fluctuation. The content of proline and soluble sugar was higher than blank control group at 72 h and 48h respectively and soluble protein was lower than blank control group under 0℃. Exogenous ABA treatment, increased the antioxidant enzymes activities with different extent under low temperature stress. For the osmotic regulation substances, exogenous ABA treatment decreased the relative conductivity and increased the content of proline, soluble sugar and soluble protein.

Key words: low temperature stress, Choraspora bungeana, abscisic acid, antioxidant enzymes, osmotic regulation substances

中图分类号:

Q949.748.3

何文平, 王新霞, 张旭强, 杨鹏军, 仇奕之, 杨宁. 低温胁迫下外源脱落酸对高山离子芥抗氧化酶和渗透调节物质的影响[J]. 植物研究, 2016, 36(6): 870-877.

HE Wen-Ping, WANG Xin-Xia, ZHANG Xu-Qiang, YANG Peng-Jun, QIU Yi-Zhi, YANG Ning. Exogenous ABA Affected on Antioxidant Enzymes and Osmotic Adjustment in Choraspora bungeana Under Low Temperature Stress[J]. Bulletin of Botanical Research, 2016, 36(6): 870-877.

参考文献

1. 王向辉.西北地区环境变迁与农业可持续发展研究[D].杨凌:西北农林科技大学,2012. Wang X H.Environmental change and sustainabal development of agriculturalin northwestern china[D].Yangling:Northwest A&F University,2012.
2. 曹慧明,史作民,周晓波,等.植物对低温环境的响应及其抗寒性研究综述[J].中国农业气象,2010,31(2):310-314. Cao H M,Shi Z M,Zhou X B,et al.A review on response of plant to low temperature and it scold resistance[J].Chinese Journal of agrometeorolog,2010,31(2):310-314.
3. 罗正荣.植物激素与抗寒力的关系[J].植物生理学通讯,1989(3):1-5. Luo Z R.Relationship between plant hormones and cold resistance[J].Plant Physiology Communications,1989(3):1-5.
4. Jonesa M.A new look at stress:abscisic acid patterns and dynamics at high-resolution[J].New Phytologist,2016,210(1):38-44.
5. de Zelicourt,Colcombet J,Hirt H.The Role of MAPK Modules and ABA during Abiotic Stress Signaling[J].Trends in plant science,2016,21(8):677-685.
6. Prasad T K,Anderson M D,Martin B A,et al.Stewart R.Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen-peroxide[J].Plant Cell,1994,6(1):65-74.
7. Tajeddine N.How do reactive oxygen species and calcium trigger mitochondrial membrane permeabilisation?[J].Biochimica et Biophysica Acta,2016,1860(6):1079-1088.
8. Mittova V,Tal M,Mvolokita M,et al.Salt stress induces up-regulation of an effect chloroplast antioxidant system in the salt-toler wild tomato species Lycopersicon pennellii but not in the cultivated species[J].Physiologia Plantarum,2002,115(3):393-400.
9. Mittler R,Vanderauwera S,Gollery M,et al.Reactive oxygen gene network of plants[J].Trends in Plant Science,2004,9(10):490-498.
10. Singh S,Anjum Na,Khan Na,et al.Metal-binding peptides and anti-oxidant defence system in plants:significance in cadmium tolerance[M].//Khan,Singh S.Abiotic Stress and Plant Responses.New Delhi:IK International,2008:159-189.
11. 王娟,李德全.逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J].植物学通报,2001,18(4):459-465. Wang J,Li D Q.The accumulation of Plant osmoticum and activated oxygen metabolism under stress[J].Chinese Bulletin of Botany,2001,18(4):459-465.
12. 杨宁,王程亮,陈霞,等.干旱胁迫对高山离子芥试管苗抗氧化系统及其活性氧代谢的影响[J].西北植物学报,2014,34(12):2483-2490. Yang N,Wang C L,Chen X,et al.Effect of drought stress on antioxidant system and reactive oxygen in Chorispora bungeana plantlets in vitro[J].Acta Bot.Boreal.-Occident.Sin,2014,34(12):2483-2490.
13. 白慕群.高山离子芥低温诱导蛋白的鉴定和功能研究[D].兰州:兰州大学,2012. Bai M Q.Identification and characterization of low temperature induced proteins in Chorispora bungeana[J].Lanzhou:Lanzhou university,2012.
14. 杨宁,丁芳霞,王程亮,等.低温胁迫对高山离子芥试管苗渗透调节物质及保护酶系的影响[J].四川大学学报:自然科学版,2014,51(2):368-390. Yang N,Ding F X,Wang C L,et al.Effect of low temperature stress onosmotica and protective enzyme system in chorispora bunggeana plantlets in vitro[J].Journal of Sichuan University:Natural Science,2014,51(2):368-390.
15. Rajinder S.Leaf senescence,cor-related with increased levels of membrane permeability and lipid peroxidation,and decreased levels of superoxide,dismutase and catalase[J].Jonrnal of Experimental botany,1981,32(126):93-101.
16. Amako,Katsumi,Chen G X,et al.Separare assays specific for ascordate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymse of ascarbate peroxidase in plants[J].Plants & Cell Physiology,1994,35(3):497-504.
17. 李仕飞,刘世同,周建平,等.分光光度法测定植物过氧化氢酶活性的研究[J].安徽农学通报,2007,13(2):72-73. Li S F,Liu S T,Zhou J P,et al.Measuration of catalase vigor in plants with spectrophotometry[J].Anhui Agricultural Science Bulletin,2007,13(2):72-73.
18. 李忠光,李江鸿,杜朝昆,等.在单一提取系统中同时测定五种植物抗氧化酶[J].云南师范大学学报,2002,22(6):44-48. Li Z G,Li J H,Du C K,et al.Simultaneous measurement of five antioxidant enzyme activities using a single extraction system[J].Journal of Yunnan Normal University,2002,22(6):44-48.
19. 郝建军,康宗利,于洋.植物生理学实验技术[M].北京:化学工业出版社,2006. Hao J J,Kang Z L,Yu Y.plant physiology Experimental technology[M].Beijing:Chemical industry Press,2006.
20. 张宪政.植物生理学实验技术[M].沈阳:辽宁科技出版社,1989. Zhang X Z.plant physiology Experimental technology[M].Shenyang:Liaoning science and technology publishing house,1989.
21. 杨颖丽,杨宁,王莱,等.盐胁迫对小麦幼苗生理指标的影响[J].兰州大学学报:自然科学版,2007,43(2):29-34. Yang Y L,Yang N,Wang L,et al.Effect of salinity on the physiological characteristics in two wheat cultivars[J].Journal of Lanzhou University:Natural Science,2007,43(2):29-34.
22. Bates L S,Waldren R P,Teare I D.Rapid determination of free proline for water stress studies[J].Plant and Soil,1973,39(1):205-207.
23. 李璇,岳红,王升,等.影响植物抗氧化酶活性的因素及其研究热点和现状[J].中国中药杂志,2013,38(7):973-978. Li X,Yue H,Wang S,et al.Research of different effects on activity of plant antioxidant enzymes[J].China Journal of Chinese Material Medica,2013,38(7):973-978.
24. 李泽琴,李静晓,张根发.植物抗坏血酸过氧化物酶的表达调控以及对非生物胁迫的耐受作用[J].遗传,2013,35(1):45-54. Li Z Q,Li J X,Zhang G F.Expression regulation of plant ascorbate peroxidase and its tolerance to abiotic stresses[J].Hereditas,2013,35(1):45-54.
25. 李雪梅,陈强,王兰兰,等.脱落酸对小麦幼苗光合及抗氧化酶的影响[J].沈阳师范大学学报:自然科学版,2006,24(2:):221-223. Li X M,Chen Q,Wang L L,et al.Effects of abscisic acid on photosynthesis and antioxidant enzymes in wheat seedling[J].Journal of S henyang Normal University:Natural Science,2006,24(2):221-113.
26. 黄杏,梁勇生,杨丽涛,等.低温胁迫下脱落酸及合成抑制剂对甘蔗幼苗抗氧化系统的影响[J].华南农业大学学报,2013,34(3):356-361. Huang X,Liang Y S,Yang L T,et al.Effects of abscisic acid and its biosynthesis inhibitor on the activities of antioxidant system of sugarcanes treated by cold stress[J].Journal of South China agricultural university,2013,34(3):356-361.
27. 罗立津,徐福乐,翁华钦,等.脱落酸对甜椒幼苗抗寒性的诱导效应极其机理研究[J].西北植物学报,2011,31(1):94-100. Luo L J,Xu F L,Weng H Q,et al.Inducing effects and its biological mechanisms of ABA on the chilling resistance of sweet pepper seedlings[J].Acta Bot.Boreal.-Occident.Sin,2011,31(1):94-100.
28. 刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社,1994. Liu Z Q,Zhang S C.Plant resistance physiology[M].Beijing:China Agriculture Press,1994.
29. Chen T H H,Gusta L V.Abscisic acid-induced freezing resistance in cultured plant cells[J].Plant Physiology,1983,73(1):71-5.
30. 罗焕亮,徐位力,李建忠,等.马占相思对低温胁迫适应性的研究[J].华南农业大学学报:自然科学版,2002,23(2):51-53. Luo H L,Xu W L,Li J Z,et al.Study on the acceptation of acacia mangium to low temperature stress[J].Journal of South China Agricultural University:Natural Science Edition,2002,23(2):51-53.
31. Kishor P B K,Sangam S,Amrntha R,et al.Regulation of proline biosynthesis,degradation,uptake and transport in higher plants:Its impli-cations in plant growth and abiotic stress tolerance[J].Current Science,2004,65(8):2145-2156.
32. 邵红雨,孔广超,齐军仓,等.植物耐盐生理生化特性的研究进展[J].安徽农学通报,2006,12(9):51-53. Shao H Y,Kong G C,Qi J C,et al.Advances in research on physiological and characteristics of salt tolerance in plant[J].Anhui Agricultural Science Bulletin,2006,22(9):51-53.
33. 汤章成.现代植物生理学实验指南[M].北京:科学出版社,2004. Tang Z C.Guide of modern plant physiology experiment[M].Beijing:Science Press,2004.
34. Schaff T,Levin L,Blair N,et al.Spatial heterogeneity of benthos on the Carolina continental slope:large(100k) scalbnvv b e variation[J].Marine Ecology progress,1992,88(2-3):143-160.
35. 孙庆玲,李培英,孙宗玖,等.外施脱落酸对不同抗寒性狗牙根品种的渗透调节物质响应研究[J].新疆农业大学学报,2012,35(2):87-92. Sun Q L,Li P Y,Sun Z J,et al.studies on re sponse of spraying exogenous ABA to osmotic adjustment substance of bermudagrass varieties with different cold resistan ce[J].Journal of Xinjiang Agricultural University,2012,35(2):87-92.
36. 李冬梅,韩小强,于俊华,等.外源脱落酸缓解低温胁迫研究进展[J].现代农药,2016,15(4):1-5. Li D M,Han X Q,Yu J H,et al.Research advances in chilling resistance of exogenous abscisic acid[J].Modern Agrochemicals,2016,15(4):1-5.

[1]	刘森尧, 贾丰璘, 国庆, 樊高锋, 周博如, 姜廷波. 小黑杨转录因子PsnbHLH162基因在盐和低温胁迫下应答分析[J]. 植物研究, 2023, 43(2): 300-310.
[2]	黄东梅, 陈颖, 白露, 倪迪安, 徐奕扬, 张志国, 秦巧平. 萱草叶片响应低温胁迫的转录组分析[J]. 植物研究, 2022, 42(3): 424-436.
[3]	代雅琦, 刘艳萍, 韩路, 王海珍. 地下水埋深对胡杨叶片光合作用及抗氧化物质积累的影响[J]. 植物研究, 2022, 42(2): 299-308.
[4]	彭春雪, 崔雪梅, 沈海龙. 暴马丁香成熟胚愈伤组织和体胚诱导及其生理状态解析[J]. 植物研究, 2021, 41(4): 557-563.
[5]	胡馨丹, 李瑶, 张小花, 梁娟红, 张腾国. 外源ATP对油菜幼苗耐寒性的影响[J]. 植物研究, 2021, 41(2): 302-311.
[6]	鲁琳, 杨尚谕, 刘维东, 鲁黎明. 基于转录组测序的花烟草低温胁迫响应转录因子挖掘[J]. 植物研究, 2021, 41(1): 119-129.
[7]	王宁, 董莹莹, 袁美丽, 王亚楠, 曾胜蕴. 温水浸种对杜仲种子萌发过程中保护酶活性及内源激素含量变化的影响[J]. 植物研究, 2020, 40(4): 523-529.
[8]	关韬, 刘超, 李开隆, 夏德安, 马旭俊. 杨树HDA902基因在低温胁迫应答反应中的功能[J]. 植物研究, 2020, 40(2): 251-256.
[9]	樊松乐, 王纪坤, 谢贵水, 王萌, 王立丰. R2R3型MYB转录因子HbMYB88结构和功能分析[J]. 植物研究, 2020, 40(1): 106-116.
[10]	刘建新, 欧晓彬, 王金成. 外源H₂O₂对盐碱混合胁迫下裸燕麦幼苗生长和抗性生理的影响[J]. 植物研究, 2019, 39(2): 181-191.
[11]	彭春雪, 崔雪梅, 刘春苹, 杨玲, 王秋水, 沈海龙. 3种抗褐化剂影响水曲柳体胚发生的生理分析[J]. 植物研究, 2019, 39(2): 252-258.
[12]	赵敏, 王玥萱, 徐运飞, 赵启安, 刘博, 杨宁. 干旱胁迫下拟南芥中H₂S与ABA信号关系研究[J]. 植物研究, 2019, 39(1): 104-112.
[13]	赵敏, 赵启安, 刘博, 王玥萱, 张莉环, 杨宁. H₂S对干旱胁迫下高山离子芥的作用研究[J]. 植物研究, 2018, 38(6): 902-912.
[14]	吴敏怡, 李霞, 何亚飞, 张琛, 严婷. 脱落酸和己糖激酶抑制剂对高表达C₄-PEPC转基因稻苗耐旱性的影响[J]. 植物研究, 2017, 37(3): 402-415.
[15]	刘建新1,2；王金成1,2；王瑞娟1,2；贾海燕1,2. 混合盐碱胁迫下裸燕麦的种子萌发和幼苗逆境生理特征[J]. 植物研究, 2016, 36(2): 224-231.