H2S对干旱胁迫下高山离子芥的作用研究

doi:10.7525/j.issn.1673-5102.2018.06.014

摘要/Abstract

摘要： 以高山冰缘植物高山离子芥（Chorispora bungeana）试管苗为实验材料，研究了0.3 mol·L^-1甘露醇模拟干旱胁迫响应过程中硫化氢（H₂S）调节高山离子芥的膜系统损伤程度、渗透调节物质和抗氧化酶系的作用，以及磷脂酶D（PLD）、活性氧（ROS）与H₂S信号分子在高山离子芥中响应干旱胁迫中的作用和可能存在的信号关系。结果显示：干旱胁迫下，外施H₂S供体NaHS显著降低高山离子芥电解质渗漏率及MDA含量、抑制ROS产生，提高渗透调节物质和抗氧化水平，从而增强高山离子芥的抗旱能力；干旱可诱导PLD活性、H₂S含量、ROS发生显著变化；当分别外施PLD下游产物PA与ROS供体H₂O₂均可促进干旱胁迫下H₂S的释放，当同时外施PA和ROS抑制剂DPI时对干旱胁迫下H₂S含量没有显著影响，当同时外施PLD抑制剂正丁醇与ROS抑制剂DPI则显著抑制干旱胁迫下H₂S含量的产生，表明干旱胁迫下，高山离子芥中ROS位于PLD的下游、H₂S的上游发挥作用。

关键词: 高山离子芥, 干旱胁迫, 硫化氢, 信号关系

Abstract: We used the plantlets of Chorispora bungeana, which wasan alpine subnival plant species,to study the membrane system damage induced by exogenous hydrogen sulfide (H₂S) during the response of mannitol (with the concentration of 0.3M) to simulate drought stress. We studied the contents of osmotic adjustment substances, the activities of antioxidant enzymes system, the phospholipase D (PLD), reactive oxygen species (ROS) and signaling molecule H₂S in response to drought stress, and the possible signaling relationship of PLD,ROS and H₂S was also discussed. NaHS, the exogenous H₂S donor, significantly reduced the electrolyte leakage rate and MDA content,enhanced the osmotic adjustment substances and the activities of antioxidant enzymes, suppressed the production of ROS, and improved the drought resistance of C.bungeana. The PLD activity, H₂S content and ROS could be induced significantly under drought stress.Under drought stress, the release of H₂S can be promoted significantly with applying the PA (downstream product of PLD) and H₂O₂ (ROS donor) separately. However, there was no significant effect on H₂S content under drought stress with adding DPI (Both PA and ROS inhibitor) simultaneously. The production of H₂S was significantly inhibitedunder drought stress,when 1-butanol (PLD inhibitor) and DPI (ROS inhibitor) were simultaneously applied. Therefore, ROS were located at the upstream of PLD and downstream of H₂S in C.bungeana under drought stress.

Key words: Chorispora bungeana, drought stress, hydrogen sulfide, signal relationship

中图分类号:

Q949.748.3

赵敏, 赵启安, 刘博, 王玥萱, 张莉环, 杨宁. H₂S对干旱胁迫下高山离子芥的作用研究[J]. 植物研究, 2018, 38(6): 902-912.

ZHAO Min, ZHAO Qi-An, LIU Bo, WANG Yue-Xuan, ZHANG Li-Huan, YANG Ning. Effect of H₂S on Chorispora bungeana under Drought Stress[J]. Bulletin of Botanical Research, 2018, 38(6): 902-912.

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H₂S对干旱胁迫下高山离子芥的作用研究

Effect of H₂S on Chorispora bungeana under Drought Stress

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