不同植物中推定蓖麻烯合酶基因的生物信息学分析

doi:10.7525/j.issn.1673-5102.2016.04.017

植物研究 ›› 2016, Vol. 36 ›› Issue (4): 605-612.doi: 10.7525/j.issn.1673-5102.2016.04.017

不同植物中推定蓖麻烯合酶基因的生物信息学分析

刘洪伟¹, 杨艳芳¹, 熊王丹², 吴平治², 吴国江², 邱德有¹

1. 林木遗传育种国家重点实验室, 中国林业科学研究院林业研究所, 北京 100091;
2. 中国科学院华南植物园植物资源保护与可持续利用重点实验室, 广州 510650

收稿日期:2016-01-13 出版日期:2016-07-15 发布日期:2016-06-15
通讯作者: 邱德有,E-mail:qiudy@caf.ac.cn E-mail:qiudy@caf.ac.cn
作者简介:刘洪伟(1987-),男,博士研究生,主要从事植物次生代谢方向的研究。
基金资助:
国家自然科学基金(31270705);中国林业科学研究院林业研究所中央级公益性科研院所基本科研业务费专项(RIF2014-01)

Bioinformatics Analysis of Casbene Synthase Genes in Different Plants

LIU Hong-Wei¹, YANG Yan-Fang¹, XIONG Wang-Dan², WU Ping-Zhi², WU Guo-Jiang², QIU De-You¹

1. State Key Laboratory of Forest Tree Genetics and Breeding, the Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091;
2. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650

Received:2016-01-13 Online:2016-07-15 Published:2016-06-15
Supported by:
The National Natural Science Foundation of China(31270705);National non-profit Research Institutions of Chinese Academy of Forestry(RIF2014-01)

摘要/Abstract

摘要： 利用GenBank中已登录的完整的麻风树、乳浆大戟、蓖麻和乌桕中的13个蓖麻烯合酶(Casbene synthase,CS;EC 4.6.1.7)基因序列,通过生物信息学方法对其核酸及氨基酸序列、组成成分、导肽、信号肽、跨膜结构域、疏水性/亲水性、蛋白质的二级结构、三级结构及功能域等进行了分析预测。结果表明,13个CS基因的ORF长度均在1647~1845 bp,蛋白分子量均在63.0~70.8 kD,终止密码子为TGA或TAA,理论等电点均小于7.0,表明CS蛋白呈酸性。氨基酸含量最高的均为亮氨酸。核苷酸同源性比较分析表明,CS基因主要分为两类。导肽预测发现其中6个CS具有导肽,均为叶绿体导肽。信号肽和扩模结构域预测发现这些CS不存在信号肽和跨膜结构域,肽链整体呈现为亲水性。这些CS的主要二级结构元件为α-螺旋,并且都包含两个萜类合酶功能域。以上研究为进一步探索CS基因的功能提供一定理论依据。

关键词: 巴豆烷, 蓖麻烯合酶, 生物信息学

Abstract: We used bioinformatics to study 13 casbene synthase (CS; EC 4.6.1.7) full-length gene sequences registered in the GenBank from Euphorbia esula, Jatropha curcas L., Ricinus communis and Triadica sebifera, and predicted the composition of nucleic acid and amino acid sequences, leader peptides, signal peptide, trans-membrane topological structure, hydrophobicity or hydrophilicity, the secondary and tertiary structure as well as the function domains. The 13 genes encoding ORFs were 1647-1845 bp, the molecular weight of the 13 predicted proteins were 63.0-70.8 kD, and the termination codons were TGA or TAA. The theoretical isoelectric points of the 13 proteins were lower than 7.0, which suggested that CS proteins were acidic. Leu was the most contented amino acid. By the homologous alignment of nucleic acid, CS genes were divided into two groups. The prediction of leading peptides showed that at least 6 CSs had leader peptide (chloroplast leader peptide mainly) in common. All 13 CSs had no signal peptide and trans-membrane topological structure in and the peptide chains were hydrophilicity. α-helix was the dominant secondary structure constructional element of the 13 proteins which contained two terpenoid synthases function domains.

Key words: tigliane, casbene synthase, bioinformatics

中图分类号:

S565.6

刘洪伟, 杨艳芳, 熊王丹, 吴平治, 吴国江, 邱德有. 不同植物中推定蓖麻烯合酶基因的生物信息学分析[J]. 植物研究, 2016, 36(4): 605-612.

LIU Hong-Wei, YANG Yan-Fang, XIONG Wang-Dan, WU Ping-Zhi, WU Guo-Jiang, QIU De-You. Bioinformatics Analysis of Casbene Synthase Genes in Different Plants[J]. Bulletin of Botanical Research, 2016, 36(4): 605-612.

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不同植物中推定蓖麻烯合酶基因的生物信息学分析

Bioinformatics Analysis of Casbene Synthase Genes in Different Plants

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