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植物研究 ›› 2015, Vol. 35 ›› Issue (3): 457-461.doi: 10.7525/j.issn.1673-5102.2015.03.021

• 论文 • 上一篇    下一篇

一种简易高效提取多种植物纯净DNA的方法

丁浩;郑唐春;梁德洋;曲冠证*   

  1. 东北林业大学林木遗传育种国家重点实验室,哈尔滨 150040
  • 出版日期:2015-05-20 发布日期:2015-06-24
  • 基金资助:
     

A Simple and Efficient Method of DNA Extraction from Different Plant Samples

DING Hao;ZHENG Tang-Chun;LIANG De-Yang;QU Guan-Zheng*   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Online:2015-05-20 Published:2015-06-24
  • Supported by:
     

摘要: 植物组织中次生代谢产物较多,要从中快速提取高质量的DNA比较困难。本文利用一种改良的CTAB法,通过在裂解后直接添加RNase A去除RNA污染,然后再经过一系列的抽提、沉淀、洗涤,获得纯净的DNA。经用琼脂糖凝胶电泳及核酸检测仪检测总DNA的纯度、浓度及质量。以提取的DNA为模版,PCR能够获得清晰的目的条带。结果表明通过该法可以简易、快速、高通量的提取多种植物的纯净DNA,为后续的分子生物学分析奠定了技术基础。

关键词: 植物, 方法改良, DNA提取, PCR

Abstract: It is difficult to extract high quality DNA from plants for molecular work, since there are a great deal of secondary metabolities. We provided a simple improved CTAB protocol for extracting plant DNA. RNA was digested with RNase A after the step of incubation at 65℃. Then, DNA was extracted, precipitated and washed. The purity and quality of total DNA was identified by agarose gel electrophoresis and nucleic acid detection instrument. PCR amplification of the extracted DNAs as templates could present clear bands of the target gene. Compared with the previous methods, it was simple and high throughput to be used for extraction of purified DNA from different plant samples, which would set a technical foundation of subsequent analysis of molecular biology.

Key words: plant, improved method, extraction of DNA, PCR

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