关键词: 香蕉, 巴西橡胶, cat基因, 克隆, 序列分析

Abstract: According to the existence of plant cat genes in GenBank, we designed a pair of degenerate primers, and amplified cat genes of banana, Hevea brasiliensis, yellow lantern pepper, pineapple, sugar cane, papaya from Hainan island. Ultimately, we cloned the rubber cat-1 and banana cat-2 genes, while the others not. Sequence analysis showed that open reading frame (ORF) of rubber cat-1 and banana cat-2 genes were both 1 479 bp, and encoded 492 amino acids. GenBank accession number were HQ660587 and HQ660588. Using bioinformatics software, the tertiary structures of rubber’s CAT-1 and banana’s CAT-2 proteins were predicated, which were similar to Exiguobacteriu Oxidotolerans (PDB code：2j2mA0) and Pseudomonas Syringae (PDB code：1m7sA), respectively. Phylogenetic analysis indicated that Hainan H.brasiliensis CAT-1 possessed the highest homology with Ricinus communis CAT-1 (XP_002521709.1), while banana CAT-2 was the highest homology with Elaeis guineensis CAT-2 (ACF06566.1).

Key words: Banana, Hevea brasiliensis, cat gene, cloning, sequence analysis