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植物研究 ›› 2010, Vol. 30 ›› Issue (4): 428-433.doi: 10.7525/j.issn.1673-5102.2010.04.009

• 论文 • 上一篇    下一篇

不同激素及其浓度配比对深山蔷薇嫩叶柄再生植株诱导的影响

顾地周1;高捍东2*;王玉方3;冯颖1   

  1. 1.通化师范学院生物系,通化 134002;2.南京林业大学森林资源与环境学院,南京 210037;3.农业部特种经济动植物及产品质量监督检验测试中心,吉林 132109
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-20 发布日期:2010-07-20
  • 通讯作者: 高捍东
  • 基金资助:
     

Effect of Various Hormones with Different Concentration Ratios on the Regeneration Plant from Tender Petioles of Rosa marretii Levl.

GU Di-Zhou;GAO Han-Dong*;WANG Yu-Fang;FENG Ying   

  1. 1.Department of Biology,Tonghua Normal University,Tonghua 134002;2.College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037;3.Supervision and Testing Center for Special Economic Animal and Plant Product Quality Ministry of Agriculture P.R.China,Jilin 132109
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20
  • Contact: GAO Han-Dong
  • Supported by:
     

摘要: 以深山蔷薇新生嫩叶柄为外植体,应用均匀设计法筛选其最适合的愈伤组织诱导、愈伤组织芽苗分化和生根的培养基,结果表明,最适合的嫩叶柄愈伤组织诱导的培养基为:LS+6-BA 1.80 mg·L-1+NAA 0.10 mg·L-1+2,4-D 0.08 mg·L-1,诱导率为99.0%;愈伤组织再分化芽苗的培养基为:LS+6-BA 3.45 mg·L-1+NAA 0.05 mg·L-1,分化率为99.5%;生根培养基:1/2LS(大量元素)+IAA 0.05 mg·L-1,生根率达98.0%以上。移栽成活率达96.0%以上。本试验成功建立了深山蔷薇嫩叶柄植株再生体系。

关键词: 深山蔷薇, 叶柄, 愈伤组织, 再生植株, 均匀设计

Abstract: The tender petioles of Rosa marretii Levl. was used as explant. Uniform design was used for selecting the most suitable media for callus induction from tender petioles, callus redifferentiation and regeneration shoots rooting. The results showed that LS+6-BA 1.80 mg·L-1+NAA 0.10 mg·L-1+2,4-D 0.08 mg·L-1 was suitable for callus induction with the induction rate of callus being 99.0%; LS+6-BA 3.45 mg·L-1+NAA 0.05 mg·L-1 was suitable for callus redifferentiation with the differentiation rate of callus being 99.5%; 1/2LS(macro-elemenyts)+IAA 0.05 mg·L-1 was suitable for rooting with the rate of rooting being more than 98.0%.Transplant success ratio was more than 96.0%. The experiment results proved that plant regeneration system has been successfully established from the tender petioles of R.marretii Levl..

Key words: Rosa marretii Levl., petiole, callus, regeneration plant, uniform design

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