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植物研究 ›› 2013, Vol. 33 ›› Issue (3): 346-350.doi: 10.7525/j.issn.1673-5102.2013.03.015

• 论文 • 上一篇    下一篇

广西野生桃金娘SRAP体系优化及建立

邱文武1;韦持章1;郭凌飞2;杨祥燕3;覃振师1;王文林1*   

  1. 1.广西橡胶研究所 龙州 532415;2.厦门市农业机械监理所,厦门 361009;3.广西壮族自治区亚热带作物研究所,南宁 530001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-05-20 发布日期:2013-05-20
  • 通讯作者: 王文林
  • 基金资助:
     

Optimization of SRAP System for Guangxi Wild Rhodomyrtus tomentosa(Ait.) Hassk.

QIU Wen-Wu;WEI Chi-Zhang;GUO Ling-Fei;YANG Xiang-Yan;QIN Zhen-Shi;WANG Wen-Ling*   

  1. 1.Guangxi Rubber Research Institute,Longzhou 532415;2.Xiamen Agricultural Machinery Supervision Institute,Xiamen 361009;3.Guangxi Subtropical Crops Research Institute,Nanning 530001
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-05-20 Published:2013-05-20
  • Contact: WANG Wen-Ling
  • Supported by:
     

摘要: 利用均匀设计方法优化了桃金娘SRAP反应体系。得出最佳反应体系,其总反应体积为25 μL,包括2.5 μL 10×PCR buffer,1.0U Taq DNA聚合酶,20 ng模板DNA,0.2 mmol·L-1 dNTPs,0.3 μmol·L-1引物,2.5 mmol·L-1 Mg2+。采用优化的扩增体系,以Me1-Em11引物组合对8个参试种质进行SRAP扩增,扩增出的条带清晰、多态性好。所确立的体系稳定可靠,适于进行桃金娘的SRAP遗传分析。

关键词: 桃金娘, 均匀设计, SRAP, 体系优化

Abstract: By the method of uniform design, the reaction system for SRAP marker in Rhodomyrtus tomentosa(Ait.) Hassk. was established and optimized. The results showed that an optimal 25 μL reaction system of SRAP for R.tomentosa included 2.5 μL 10×PCR buffer, 1.0 U Taq DNA polymerase, 20 ng template DNA, 0.2 mmol·L-1 dNTPs, 0.3 μmol·L-1 primer and 2.5 mmol·L-1 Mg2+. This optimum reaction system was applied to fingerprint 8 varieties of R.tomentosa accessions by Me1-Em11 primers and produced clear polymorphic patterns. It showed that the optimized system could be effectively applied in the germplasm identification and genetic diversity analysis of R.tomentosa.

Key words: Rhodomyrtus tomentosa(Ait.) Hassk., uniform design, SRAP, system optimization

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