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植物研究 ›› 2017, Vol. 37 ›› Issue (4): 542-548.doi: 10.7525/j.issn.1673-5102.2017.04.009

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84K杨愈伤组织再生体系和直接分化再生体系遗传转化的比较性研究

王丽娜, 王玉成, 杨传平   

  1. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040
  • 收稿日期:2017-02-02 出版日期:2017-07-15 发布日期:2017-07-22
  • 通讯作者: 杨传平,E-mail:yangcp@nefu.edu.cn E-mail:yangcp@nefu.edu.cn
  • 作者简介:王丽娜(1979-),女,讲师,主要从事林木基因组研究。
  • 基金资助:
    中央高校基本科研业务费专项资金项目(2572016BA02);哈尔滨市应用技术研究与开发项目(青年后备人才A类)(2016RAQXJ065)资助

The Comparative study of Callus and Direct Differation Regenaration System of 84K Poplar

WANG Li-Na, WANG Yu-Cheng, YANG Chuan-Ping   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040
  • Received:2017-02-02 Online:2017-07-15 Published:2017-07-22
  • Supported by:
    Fundamental Research Funds for the Central Universities(2572016BA02);Harbin applied technology research and development project(2016RAQXJ065)

摘要: 植物转基因必须以建立良好的植物转化受体系统为基础,而良好的受体系统必须以具有高频率的转化率及较强的再生能力为前提。实验以84K杨叶片为材料研究其在不同培养条件、不同种类及浓度的植物激素对其脱分化及再分化的影响,并建立了两种成熟的组织再生系统,得出组织培养体系与遗传转化体系的受体系统在激素配比方面存在一定差距,所以在此基础上进行了PCK1、PCK2两个基因的愈伤途径和不定芽途径途径的遗传转化,并对两种遗传转化体系进行了对比性分析并总结出两种体系的优缺点。

关键词: 84K杨, 愈伤组织, 直接分化, 遗传转化

Abstract: High-frequent transformation and strong regeneration ability of plants are prerequisites to the establishment of good plant transformation receptor system, which is the foundation for plant genetic transformation. Taking 84K poplar leaves as study material, this experiment studies the influence of different types and concentrations of plant hormones to its dedifferentiation and re-differentiation under different cultivation conditions, and sets two mature plant tissue cultivation regeneration system. Based on above,here establishes genetic transformation system of adventitious buds and callus pathway, respectively as callus regeneration system and regeneration system of direct differentiation. Then through conducting comparative analysis of two tissue culture system using PCK1 and PCK2, here summarizes the advantages and disadvantages of two methods.

Key words: Poplar 84K, callus, direct differation, genetic transformation

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