欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2004, Vol. 24 ›› Issue (3): 351-356.

• 论文 • 上一篇    下一篇

基于3种香茶菜属药用植物高效液相色谱的主成分分析

方芳1, 李似姣1, 管雁1, 诸葛磊2, 郭水良1   

  1. 1. 浙江师范大学生物科学系, 金华 321004;
    2. 杭州胡庆余堂制药厂, 杭州 310016
  • 收稿日期:2003-11-14 出版日期:2004-09-15 发布日期:2016-06-14
  • 作者简介:方芳(1969-), 女, 高级实验师, 主要从事植物学、植物生理学等研究。
  • 基金资助:
    浙江省自然科学基金(301028)、浙江省金华市科委项目经费的资助。

PCA on medicinal materials of three species of Isodon based on their HPLC

FANG Fang1, LI Si-Jiao1, GUAN Yan1, ZHU Ge-Lei2, GUO Shui-Liang1   

  1. 1. Department of Biology, Zhejiang Normal University, Jinhua 321004;
    2. Huqingyutang Traditional Pharmaceutical Factory, Hangzhou 310016
  • Received:2003-11-14 Online:2004-09-15 Published:2016-06-14

摘要: 对香茶菜(Isodon amethystoides)、显脉香茶菜(I.nervosa)和大萼香茶菜(I.macrocalyx)不同产地和器官(根、茎、叶)共21个样品进行高效液相色谱分析,以出峰时间-峰面积为指标,以样品为对象进行主成分分析,比较不同样品间的差异程度。结果发现,(1)香茶菜、大萼香茶菜、显脉香茶菜在高效液相色谱上虽然有一定的差异,但这种差异并不明显;(2)基于HPLC显示的香茶菜不同种群间差异比三个种间的差异更为明显,说明不同产地对香茶菜属植物样品的植化组成有很大的影响,不同种在植化上的相似性使它们在一定程度上可以作为替代药材;(3)研究反映出基于高效液相色谱的PCA在反映不同样品植物化学组成差异程度上具有应用价值。

关键词: 香茶菜, 居群, HPLC, 主成分分析(PCA)

Abstract: The HPLC of the 21 samples of Isodon amthystoides,I.nervosa and I.macrocalyx from different regions and parts were obtained.Based on the area and time of peak,the differences of the 21 HPLC were compared by the method of Principle Component Analysis (PCA).The results showed:(1) Though there are differences among the HPLC of 8 different populations of Isodon amthystoides,I.nervosa and I.macrocalyx,the differences aren't very obvious.(2) The intraspecific differences of HPLC spectra among the populations of Isodon amthystoides were more obvious than those among the three species,which indicate that different places affect the chemical compositions and these three species could be replaced each other as Chinese medicinal drugs to some extent.(3) It is practical to apply PCA on the basis of HPLC to show the differences of plant samples.

Key words: Isodon Kudo, Population, HPLC, PCA