一种快速有效的741杨离体叶片再生芽方法

植物研究 ›› 2004, Vol. 24 ›› Issue (3): 347-350.

一种快速有效的741杨离体叶片再生芽方法

吴双秀^1,2, 吴汪黔生², 祖元刚¹

1. 东北林业大学教育部森林植物生态学实验室, 哈尔滨 150040;
2. 香港科技大学生物系, 香港九龙清水湾

收稿日期:2003-12-01 出版日期:2004-09-15 发布日期:2016-06-14
通讯作者: 祖元刚,Tel:86-451-82191517;Email:zygorl@public.hr.hl.cn E-mail:zygorl@public.hr.hl.cn
作者简介:吴双秀,(1972-), Female, Postdoctoral research associate, Research interests in plant biot echnology and molecular biology.

A new efficient method for rapid in-vitro shoot regeneration on leaf explants of Poplar 741

WU Shuang-Xiu^1,2, WU Madeline², ZU Yuan-Gang¹

1. Key Laboratory of Forest Plant Ecology of the Ministry of Education, Northeast Forestry University, Harbin 150040;
2. Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, SAR, People's Republic of China

Received:2003-12-01 Online:2004-09-15 Published:2016-06-14
Supported by:
This research has been supported by the grant of Project 973(G19990160) and part ly supported by g rant HKUS T 6117/01M from the Research Grant Council of Hong Kong.

摘要/Abstract

摘要： 在对杂交品种741杨(Populus alba(P.davidiana×P.simonii)×P.tomentosa)进行农杆菌介导法转基因的试验中发现了一种快速有效的叶片再生芽的方法。首先叶片外植体在培养基I (MS培养基添加0.5 mg/L BA和1.0 mg/L 2,4-D)上培养2~3 d,再转移到培养基SH (MS medium containing 2.0 mg/L of BA and 0.1 mg/L of NAA)上培养10 d,然后再转移到培养基II (MS medium with 0.5 mg/L of BA)上,培养大约5 d之后86.7%的叶片外植体产生的芽,每片叶片外植体(1 cm×1 cm)可产生40~50个芽。但是,如果叶片外植体在培养基I上培养的时间长于5 d,再依次转移到培养基SH和II上,则叶片会产生大量根。

关键词: 741杨, Populus alba(P.davidiana×, P.simonii)×, P.tomentosa, 芽再生, 再生

Abstract: In the experiments of Agrobacterium tumefaciens-mediated transformation of the hybrid poplar 741,Populus alba(P.davidiana×P.simonii)×P.tomentosa,we developed a rapid and efficient method of shoot regeneration from the leaf explants in-vitro on Murashige and Skoog (MS) medium.The leaf explant segments was first cultured on medium I (MS medium supplemented with 0.5 mg/L of BA and 1.0 mg/L of 2,4-D) for 2~3 days and then transferred to medium SH (MS medium containing 2.0 mg/L of BA and 0.1 mg/L of NAA) to culture for 10 days.86.7% of leaf segments produced shoots after the leaf segments were transferred from medium SH to medium II (MS medium with 0.5 mg/L of BA) for 1 week.The average shooting number was 40~50 per 1 cm×1 cm of leaf segment.However,if the leaf explants incubated on medium I longer than 5 days,they produced more roots instead of shoots.

Key words: Poplar 741, Populus alba(P. davidiana×, P. simonii)×, P. tomentosa, shooting, regeneration

吴双秀, 吴汪黔生, 祖元刚. 一种快速有效的741杨离体叶片再生芽方法[J]. 植物研究, 2004, 24(3): 347-350.

WU Shuang-Xiu, WU Madeline, ZU Yuan-Gang. A new efficient method for rapid in-vitro shoot regeneration on leaf explants of Poplar 741[J]. Bulletin of Botanical Research, 2004, 24(3): 347-350.

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