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植物研究 ›› 2001, Vol. 21 ›› Issue (4): 574-577.

• 论文 • 上一篇    下一篇

基于SOD和EST同工酶的19种苔藓植物种间关系排序分析

邵邻相, 郭水良, 陈吉娟   

  1. 浙江师范大学生命与环境科学学院, 金华 321004
  • 收稿日期:2001-07-19 出版日期:2001-12-15 发布日期:2016-06-14
  • 作者简介:邵邻相(1962-), 男, 副教授, 主要从事植物细胞生物学研究。

ORDINATION ANALYSES OF 19 SPECIES OF BRYOPHYTA BASED ON SOD AND EST ISOZYMES

SHAO Lin-xiang, GUO Shui-liang, CHENG Ji-juan   

  1. College of Life & Environmental Sciences, Zhejiang Normal University, Jinhua 321004
  • Received:2001-07-19 Online:2001-12-15 Published:2016-06-14

摘要: 采用聚丙烯酰胺凝胶电泳的方法, 获得了金华北山苔藓植物19 个种之间酯酶(EST)、超氧化物歧化酶(SOD)的同工酶酶谱。将19 个种苔藓植物的酶谱进行量化后, 采用主成分分析(PCA)方法, 比较了19 个种种间关系的差异特点。研究表明, 苔藓植物具有很高的遗传多样性, 以同工酶酶谱资料为基础, 应用主成分分析方法能够比较直观和有效地反映出苔藓植物分类群间的系统关系。

关键词: 苔藓植物, 脂酶(EST), 超氧化物歧化酶(SOD), 同工酶, 主成分分析(PCA)

Abstract: By polyacyacrylamide get electrphoresis, two isozymes:Esterase(EST)and Superode dismutase(SOD)of 15 species of Musci and 4 species of Hepaticae were studied. Zymogramatic differences of two isozymes in 19 species were numerically revealed by Principal Component Analysis (PCA). The results showed as follow:1)Bryophytes are of high genetic diversity;2)Based on isozyme data, PCA could be applied to reveal the systematic relationships among different bryophyte species in genetic level.

Key words: Bryophytes, Esterase(EST), Superode dismutase(SOD), Isozyme, Principal Component Analysis(PCA)