植物研究 ›› 2026, Vol. 46 ›› Issue (5): 913-926.doi: 10.7525/j.issn.1673-5102.2026.05.012
赵洁茹1,2, 刘燕1,2, 陈静1,2, 秦栋1,2(
), 霍俊伟1,2(
)
收稿日期:2026-03-18
出版日期:2026-09-20
发布日期:2026-10-10
通讯作者:
秦栋,霍俊伟
E-mail:dongq9876@126.com;huojunwei@neau.edu.cn
作者简介:赵洁茹(2000—),女,硕士研究生,主要从事蓝靛果栽培技术研究。
基金资助:
Jieru ZHAO1,2, Yan LIU1,2, Jing CHEN1,2, Dong QIN1,2(
), Junwei HUO1,2(
)
Received:2026-03-18
Online:2026-09-20
Published:2026-10-10
Contact:
Dong QIN, Junwei HUO
E-mail:dongq9876@126.com;huojunwei@neau.edu.cn
摘要:
植物生长调节剂的应用是调控果树生长发育和提高果实品质的有效措施。本研究为探明花期喷施不同质量浓度芸苔素(EBR)和赤霉素(GA3)对蓝靛果(Lonicera caerulea)的影响,以主栽品种‘蓝精灵’为试验材料,分析了不同处理条件下蓝靛果植株的生长发育与果实品质指标。结果表明:2种生长调节剂均可有效促进其生长发育并提升果实品质。其中,30 mg⋅L-1 GA3显著促进新梢生长,其新梢长度较CK长72.95%;50 mg⋅L-1 GA3最有利于提高果实类黄酮含量与羟自由基清除率,其分别较CK高63.06%和14.67%;75 mg⋅L-1 GA3则能最大程度地提升抗坏血酸含量与DPPH自由基清除率,其分别较CK高27.10%和11.52%。0.1 mg⋅L-1 EBR显著提高叶片叶绿素相对含量和果实硬度,其分别较CK高30.55%和27.02%;0.3 mg⋅L-1 EBR+75 mg⋅L-1 GA3组合处理在增大叶面积(较CK高72.44%)、提升果实总花色苷含量(较CK高78.54%)和总酚含量(较CK高45.40%)方面效果最佳,并使果实总抗氧化能力达到峰值。综上所述,本研究证明了花期喷施适宜质量浓度的EBR和GA3是改善蓝靛果生长发育与果实品质的有效栽培措施,为芸苔素、赤霉素在蓝靛果栽培中的应用提供了理论依据。
中图分类号:
赵洁茹, 刘燕, 陈静, 秦栋, 霍俊伟. 芸苔素与赤霉素对蓝靛果生长及品质的影响[J]. 植物研究, 2026, 46(5): 913-926.
Jieru ZHAO, Yan LIU, Jing CHEN, Dong QIN, Junwei HUO. Effects of Brassinolide and Gibberellic Acid on the Growth and Quality of Blue Honeysuckle[J]. Bulletin of Botanical Research, 2026, 46(5): 913-926.
表1
植物生长调节剂试验处理
处理 Treatment | 质量浓度 mass concentration |
|---|---|
| CK | 清水 |
| E1 | EBR 0.05 mg·L-1 |
| E2 | EBR 0.1 mg·L-1 |
| E3 | EBR 0.3 mg·L-1 |
| E4 | EBR 0.6 mg·L-1 |
| E5 | EBR 1.0 mg·L-1 |
| G1 | GA3 15 mg·L-1 |
| G2 | GA3 30 mg·L-1 |
| G3 | GA3 50 mg·L-1 |
| G4 | GA3 75 mg·L-1 |
| G5 | GA3 100 mg·L-1 |
| F1 | EBR 0.3 mg·L-1+GA3 15 mg·L-1 |
| F2 | EBR 0.3 mg·L-1+GA3 30 mg·L-1 |
| F3 | EBR 0.3 mg·L-1+GA3 50 mg·L-1 |
| F4 | EBR 0.3 mg·L-1+GA3 75 mg·L-1 |
| F5 | EBR 0.3 mg·L-1+GA3 100 mg·L-1 |
表2
不同质量浓度芸苔素、赤霉素及组合处理对蓝靛果叶片发育的影响
处理 Treatment | 叶长 Leaf length/mm | 叶宽 Leaf width/mm | 叶面积 Leaf area/mm2 |
|---|---|---|---|
| CK | 42.46±11.91e | 27.21±2.20abc | 862.29±216.59e |
| E1 | 47.03±7.85bcde | 26.50±3.00bc | 1 083.52±50.51cde |
| E2 | 50.03±4.81abcde | 30.08±3.85abc | 1 218.89±57.17abcd |
| E3 | 52.36±2.31abcde | 31.99±3.71ab | 1 324.48±19.76abc |
| E4 | 52.07±4.81abcde | 27.57±3.88abc | 1 137.71±114.24bcde |
| E5 | 45.96±6.43cde | 25.30±1.38c | 998.53±10.26de |
| G1 | 54.34±5.69abcde | 24.74±2.44c | 1 201.06±105.46abcd |
| G2 | 58.67±4.17ab | 26.31±3.66bc | 1 222.42±127.56abcd |
| G3 | 61.38±3.84a | 27.95±4.41abc | 1 316.41±117.52abc |
| G4 | 59.64±3.96a | 30.75±2.41abc | 1 383.55±154.42ab |
| G5 | 52.94±6.13abcde | 24.97±1.13c | 1 101.11±57.38bcde |
| F1 | 43.92±6.43de | 27.89±3.64abc | 1 008.12±202.45de |
| F2 | 51.19±4.81abcde | 30.37±1.81abc | 1 117.04±192.95bcde |
| F3 | 50.03±6.19abcde | 32.13±1.15ab | 1 282.50±182.84abcd |
| F4 | 57.88±2.19abc | 32.69±1.91a | 1 486.92±132.16a |
| F5 | 55.85±5.31abcd | 30.15±3.52abc | 1 290.14±265.62abcd |
表3
不同质量浓度芸苔素、赤霉素及组合处理对蓝靛果光合特性的影响
处理 Treatment | 净光合速率 Net photosynthetic rate/ (μmol·m-2·s-1) | 气孔导度 Stomatal conductance/ (mmol·m-2·s-1) | 胞间CO2摩尔分数 Intercellular CO2 mole fraction/ (μmol·mol-1) | 蒸腾速率 Transpiration rate/ (mmol·m-2·s-1) |
|---|---|---|---|---|
| CK | 9.22±1.06b | 0.07±0.03a | 187.51±9.66a | 1.67±0.47a |
| E1 | 12.86±2.23ab | 0.09±0.03a | 140.45±43.07b | 2.39±0.60a |
| E2 | 13.43±0.74a | 0.10±0.03a | 118.88±32.93b | 2.65±0.83a |
| E3 | 13.62±1.81a | 0.11±0.02a | 116.22±33.33b | 2.71±0.58a |
| E4 | 13.39±3.35a | 0.10±0.01a | 121.23±32.36b | 2.57±0.69a |
| E5 | 11.49±2.23ab | 0.08±0.02a | 143.26±9.48b | 2.25±0.77a |
| G1 | 10.74±1.02ab | 0.07±0.01a | 151.02±19.77b | 2.08±0.18a |
| G2 | 11.24±0.82 ab | 0.08±0.01a | 150.73±43.47b | 2.16±0.20a |
| G3 | 11.47±0.75 ab | 0.08±0.02a | 142.90±26.33b | 2.17±0.43a |
| G4 | 12.71±0.60 ab | 0.09±0.01a | 140.33±23.73b | 2.56±0.26a |
| G5 | 10.58±1.47 ab | 0.06±0.02a | 158.81±18.10b | 1.73±0.43a |
| F1 | 10.16±1.16 ab | 0.07±0.01a | 165.29±31.01ab | 1.73±0.24a |
| F2 | 10.55±1.39 ab | 0.07±0.01a | 159.23±28.50ab | 1.75±0.41a |
| F3 | 10.71±2.41ab | 0.07±0.03a | 158.36±21.41ab | 1.84±0.30a |
| F4 | 11.93±0.40ab | 0.09±0.03a | 126.47±36.70c | 2.15±0.10a |
| F5 | 11.00±1.69ab | 0.08±0.01a | 133.73±46.06bc | 1.94±0.63a |
表4
不同质量浓度芸苔素、赤霉素及组合处理对蓝靛果果实外观品质的影响
处理 Treatment | 硬度 Hardness/N | 横径 Horizontal diameter/mm | 纵径 Vertical diameter/mm | 果形指数 Fruit shape index | 单果质量 Fruit mass/g |
|---|---|---|---|---|---|
| CK | 1.31±0.32b | 9.00±0.46cd | 20.38±1.92d | 2.27±0.21d | 0.79±0.07b |
| E1 | 1.38±0.42ab | 9.75±0.86bc | 22.76±0.92bcd | 2.35±0.19cd | 1.01±0.07a |
| E2 | 1.66±0.31a | 9.98±0.36bc | 23.47±1.89abcd | 2.35±0.19cd | 1.02±0.09a |
| E3 | 1.42±0.28ab | 10.07±0.30b | 23.72±1.65abc | 2.36±0.19bcd | 1.11±0.03a |
| E4 | 1.39±0.29ab | 10.67±0.40b | 21.98±1.99cd | 2.06±0.22d | 1.03±0.05a |
| E5 | 1.32±0.25b | 10.21±0.69b | 21.85±1.11cd | 2.15±0.22d | 1.01±0.05a |
| G1 | 1.29±0.28ab | 8.11±0.52d | 23.43±1.08abcd | 2.90±0.26a | 0.91±0.05a |
| G2 | 1.39±0.32ab | 8.25±0.55d | 24.50±1.99abc | 2.98±0.36a | 0.95±0.00a |
| G3 | 1.61±0.26a | 8.59±0.19d | 25.29±1.69ab | 2.95±0.21a | 0.99±0.01a |
| G4 | 1.51±0.32ab | 8.30±0.40d | 25.95±2.43a | 3.13±0.22a | 0.99±0.05a |
| G5 | 1.44±0.22ab | 8.42±0.51d | 26.10±1.38a | 3.11±0.22a | 0.96±0.04a |
| F1 | 1.36±0.07ab | 8.02±0.55d | 22.32±0.83bcd | 2.80±0.22abc | 0.95±0.09a |
| F2 | 1.51±0.09ab | 8.15±0.32d | 23.01±1.32abcd | 2.82±0.10ab | 0.96±0.12a |
| F3 | 1.60±0.29a | 8.46±0.36d | 25.39±1.65ab | 3.01±0.22ab | 1.01±0.04a |
| F4 | 1.52±0.36ab | 8.29±0.44d | 23.35±0.98 abcd | 2.83±0.25a | 0.97±0.02a |
| F5 | 1.48±0.24ab | 7.99±0.65d | 22.62±1.02bcd | 2.85±0.26a | 0.87±0.03ab |
表5
不同质量浓度芸苔素、赤霉素和组合处理对蓝靛果果实内在品质的影响
处理 Treatment | 可溶性固形物含量 Soluble solid content/% | 可滴定酸度 Titratable acidity/% | 总花色苷质量分数 Total anthocyanin mass fraction/(mg·g-1) | 类黄酮质量分数 Flavonoid mass fraction/(mg·g-1) | 总酚质量分数 Total phenolic mass fraction/(mg·g-1) | 抗坏血酸质量分数 Ascorbic acid mass fraction/(mg·g-1) |
|---|---|---|---|---|---|---|
| CK | 14.73±0.06i | 0.86±0.06ab | 1.22±0.36f | 0.36±2.92d | 16.37±0.89gh | 1.35±3.37i |
| E1 | 15.43±0.06h | 0.81±0.03bcde | 1.32±0.52d | 0.41±7.72cd | 16.42±0.35gh | 1.39±2.34hi |
| E2 | 15.50±0.10h | 0.80±0.07bcdef | 1.36±0.27c | 0.42±2.10cd | 17.74±0.46fgh | 1.42±2.34gh |
| E3 | 16.13±0.06f | 0.78±0.07bcdef | 1.78±0.63a | 0.47±5.97bcd | 20.12±0.45cd | 1.53±3.91cde |
| E4 | 15.87±0.06g | 0.79±0.04bcdef | 1.47±0.43b | 0.42±5.74cd | 19.74±0.70de | 1.50±2.50def |
| E5 | 15.43±0.06h | 0.82±0.02abcd | 1.29±0.36e | 0.40±3.79cd | 16.29±1.04h | 1.44±2.61fgh |
| G1 | 16.23±0.12ef | 0.85±0.04ab | 1.98±0.28e | 0.52±4.37abc | 20.22±0.54cd | 1.45±2.88fgh |
| G2 | 16.33±0.21ef | 0.83±0.04abc | 2.10±0.13c | 0.54±3.16abc | 20.62±0.82cd | 1.57±3.59bcd |
| G3 | 16.60±0.10b | 0.79±0.04bcdef | 2.16±0.41b | 0.59±1.81a | 21.58±0.56bc | 1.63±3.51b |
| G4 | 17.07±0.06c | 0.73±0.04ef | 2.18±0.48a | 0.53±3.13abc | 22.64±0.88ab | 1.71±5.96a |
| G5 | 16.77±0.06f | 0.75±0.02def | 2.01±0.51d | 0.51±7.09abc | 21.59±0.35bc | 1.58±2.88bc |
| F1 | 16.17±0.06de | 0.90±0.026a | 1.59±0.25e | 0.53±1.12abc | 17.83±1.17fg | 1.44±5.83fgh |
| F2 | 16.40±0.17c | 0.81±0.02bcd | 1.97±0.56d | 0.56±1.20ab | 18.42±0.73ef | 1.47±3.69efg |
| F3 | 16.67±0.06c | 0.77±0.03cdef | 2.01±0.53b | 0.58±0.36a | 21.23±0.36bcd | 1.59±1.94bc |
| F4 | 17.93±0.25a | 0.73±0.02f | 2.19±0.39a | 0.54±1.86abc | 23.81±0.90a | 1.62±3.61b |
| F5 | 17.23±0.06b | 0.73±0.02ef | 1.98±0.29c | 0.52±2.02abc | 21.45±0.75bc | 1.59±3.37bc |
表6
不同质量浓度芸苔素、赤霉素和组合处理对蓝靛果果实抗氧化能力的影响
处理 Treatment | DPPH自由基清除率 DPPH radical scavenging rate/% | FRAP总抗氧化能力 FRAP total antioxidant capacity/(mmol∙L-1) | 超氧阴离子自由基清除率 Superoxide anion radical scavenging rate/% | 羟自由基清除率 Hydroxyl radical scavenging rate/% |
|---|---|---|---|---|
| CK | 88.46±1.54f | 79.54±0.40l | 57.43±0.45j | 74.26±0.75a |
| E1 | 89.87±2.12fe | 83.39±0.72k | 57.82±0.22ij | 75.08±0.29a |
| E2 | 93.72±2.50cd | 89.36±0.72i | 58.07±0.49hi | 77.42±0.33a |
| E3 | 95.26±1.82abcd | 97.51±0.33h | 59.52±0.00def | 81.23±0.50a |
| E4 | 94.49±2.89bcd | 86.12±0.49j | 58.96±0.09fg | 78.48±0.66a |
| E5 | 92.31±3.90de | 85.98±0.58j | 58.83±0.46g | 74.77±0.58a |
| G1 | 95.48±1.73abcd | 118.53±0.47e | 58.68±0.21gh | 83.61±0.42a |
| G2 | 96.11±0.44abc | 128.08±0.49bc | 60.05±0.50cd | 84.72±0.60a |
| G3 | 97.99±1.70ab | 129.03±0.30ab | 60.19±0.58bc | 85.23±0.52a |
| G4 | 98.74±0.95a | 128.95±0.54ab | 59.99±0.24cd | 84.39±0.49a |
| G5 | 95.73±1.52abcd | 127.26±0.36c | 59.88±0.22cde | 83.26±0.98a |
| F1 | 92.81±0.96cde | 99.96±0.46g | 59.34±0.17efg | 75.77±0.42a |
| F2 | 93.53±0.44cde | 119.19±0.50e | 60.82±0.23ab | 79.84±0.53a |
| F3 | 95.91±0.34abcd | 123.76±0.33d | 60.87±0.31a | 80.02±0.52a |
| F4 | 97.76±0.37ab | 129.71±0.60a | 59.80±0.32cde | 77.26±0.57a |
| F5 | 93.50±0.57cde | 116.74±0.97f | 59.10±0.24fg | 76.82±0.47a |
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