灵武长枣果实发育过程中阿拉伯半乳糖蛋白胶体金免疫分布特征

doi:10.7525/j.issn.1673-5102.2026.01.011

摘要/Abstract

摘要：

阿拉伯半乳糖蛋白（AGPs）在果实的成熟与软化中发挥重要作用，为了探讨灵武长枣（Ziziphus jujuba ‘Lingwu Changzao’）果实发育过程中AGPs的分布特征和动态变化规律，该研究使用单克隆抗体JIM13，采用胶体金免疫电镜技术，对4个不同发育时期灵武长枣果实超微结构特征及AGPs的免疫细胞分布进行分析。结果表明：使用10 nm胶体金颗粒对果实标记后，在膨大前期，韧皮部的薄壁细胞和离维管束较远的薄壁细胞内部和细胞壁上均分布有大量的胶体金颗粒，细胞内的液泡、质体等细胞器中也有胶体金颗粒的分布；在快速膨大期，筛管分子和离维管束较远的薄壁细胞的细胞壁及细胞内部均分布有大量的胶体金颗粒；在着色期，维管束韧皮部的筛管、伴胞和薄壁细胞，以及离维管束较远的薄壁细胞的细胞壁及细胞内部胶体金颗粒的分布较少；在完熟期，维管束韧皮部的伴胞及离维管束较远的薄壁细胞在细胞壁上及靠近质膜部位分布有少量的胶体金颗粒，并且细胞内部的胶体金颗粒数量较少，在囊泡、线粒体、质体及中央大液泡上分布有零星的胶体金颗粒。研究认为，果实AGPs主要分布在细胞壁上、靠近质膜的区域、细胞内部及细胞器上；不同发育时期果实AGPs的分布存在一定的差异，在膨大前期和快速膨大期细胞壁及细胞内部AGPs分布较多，在着色期和完熟期细胞壁及细胞内部AGPs分布的数量较少。上述结果为揭示AGPs对果实品质的影响提供细胞学依据。

关键词: 灵武长枣, 果实, 阿拉伯半乳糖蛋白, 胶体金免疫电镜

Abstract:

Arabinogalactan proteins（AGPs） play an important role in fruit ripening and softening. In order to explore the distribution characteristics and dynamic changes of AGPs in fruits of Ziziphus jujuba ‘Lingwu Changzao’ during development， the monoclonal antibody JIM13 and colloidal gold immunoelectron microscopy technology were used to analyze the ultrastructural characteristics and the immunological cell distribution of AGPs of Ziziphus jujuba ‘Lingwu Changzao’ fruits at four different developmental stages. The results showed that after labeled with 10 nm colloidal gold particles， there were a large number of gold particles distributed in the cell interior and cell walls of parenchymal cells in the phloem and far away from the vascular bundles， and there were also gold particles distributed in the vacuoles， plastids and other organelles during the early stage of fruit enlargement. During the rapid enlargement period， there were a large number of gold particles distributed in the cell walls and cell interior of sieve tube molecules and parenchymal cells far away from the vascular bundles. During the coloring period， there were a few gold particles in the cell walls and cell interior of sieve tubes， companion cells and parenchymal cells in phloem of vascular bundles， as well as parenchymal cells far away from the vascular bundles. During the maturation period， in the companion cells in phloem of vascular bundles， as well as parenchymal cells far away from vascular bundles， there were a small number of gold particles on the cell walls and close to the plasma membrane， and a few gold particles inside the cell wall， and on the vesicles， mitochondria， plastids and large central vacuoles. The results suggested that AGPs were mainly distributed on the cell wall， near the plasma membrane， inside the cell and the organelles， and there were some differences in distribution of AGPs in fruits at different developmental stages： more AGPs distributed in the cell wall and inside the cell in the early bulking period and the rapid enlargement period， and a small number of AGPs distributed in the cell wall and inside the cell in the coloring and ripening periods. The results provided cytological basis for revealing the effect of AGPs on fruit quality.

Key words: Ziziphus jujuba ‘Lingwu Changzao’, fruit, arabinogalactan proteins, colloidal gold immunoelectron microscopy

中图分类号:

Q942.6

马小玲, 章英才, 王静, 郑国琦. 灵武长枣果实发育过程中阿拉伯半乳糖蛋白胶体金免疫分布特征[J]. 植物研究, 2026, 46(1): 120-130.

Xiaoling MA, Yingcai ZHANG, Jing WANG, Guoqi ZHENG. The Colloidal Gold Immune Distribution Characteristics of Arabinogalactan Proteins during Fruit Development of Ziziphus jujuba ‘Lingwu Changzao’[J]. Bulletin of Botanical Research, 2026, 46(1): 120-130.

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