植物研究 ›› 2020, Vol. 40 ›› Issue (6): 876-885.doi: 10.7525/j.issn.1673-5102.2020.06.010
付涛1, 王志龙1(), 林乐静1, 林立1, 李文1, 袁冬明2
收稿日期:
2019-11-19
出版日期:
2020-11-20
发布日期:
2020-11-04
通讯作者:
王志龙
E-mail:wangzhl01@163.com
作者简介:
付涛(1988—),男,硕士,实验师,主要从事植物育种研究。
基金资助:
Tao FU1, Zhi-Long WANG1(), Le-Jing LIN1, Li LIN1, Wen LI1, Dong-Ming YUAN2
Received:
2019-11-19
Online:
2020-11-20
Published:
2020-11-04
Contact:
Zhi-Long WANG
E-mail:wangzhl01@163.com
About author:
FU Tao(1988—),male,M.A,experimentalist,major in plant breeding research.
Supported by:
摘要:
以88份樱花为试验材料,利用核基因ITS和叶绿体间隔序列trnH-psbA进行测序,经过软件MEGA 6.0处理后,构建了系统发育树。结果表明,山樱群樱花品种系统发育比较混乱,这与其遗传背景比较复杂以及原始亲本系统发育关系较近有关,江户彼岸群和寒绯樱群的大多数品种能够聚类在一起,与山樱群品种能分得开,但涉及到很多杂交品种,ITS和trnH-psbA难以区分,表明DNA条形码技术难以应用到杂交品种的系统发育关系,此外,还可依据某些樱花品种特异的ITS或trnH-psbA序列长度对部分品种进行分子鉴定,也对部分种系不明的品种进行了修订。
中图分类号:
付涛, 王志龙, 林乐静, 林立, 李文, 袁冬明. 中国主栽樱花的分子系统发育分析[J]. 植物研究, 2020, 40(6): 876-885.
Tao FU, Zhi-Long WANG, Le-Jing LIN, Li LIN, Wen LI, Dong-Ming YUAN. Molecular Phylogenetic Analysis of Cerasus Plants in China[J]. Bulletin of Botanical Research, 2020, 40(6): 876-885.
表1
样品信息
序号No. | 物种Species | 来源Source |
---|---|---|
山樱群Cerasus serrulata group | ||
1 | 青肌樱Cerasus Multiplex | 原产中国 Native to China |
2 | 仙台屋樱C.jamasakura‘Sendaiya’ | 红山樱系园艺栽培品种Horticultural cultivar of C.jamasakura series |
3 | 内里樱C.jamasakura‘Imperialis’ | 红山樱系园艺栽培品种Horticultural cultivar of C.jamasakura series |
4 | 市原虎尾樱C.jamasakura‘Albo Plena’ | 红山樱系园艺栽培品种Horticultural cultivar of C.jamasakura series |
5 | 红叶樱C.serrulata‘Hongye’ | 山樱系园艺栽培品种Horticultural cultivar of C.serrulata series |
6 | 奈良八重樱C.verecunda‘Antiqua’ | 霞樱系重瓣品种Double variety of C.verecunda series |
7 | 大岛樱C.speciosa | 日本原生种 Japanese native species |
8 | 伊豆樱C.speciosa‘Izuzakura’ | 大岛樱变异品种Variation variety of C.speciosa |
9 | 赤实大岛樱C.speciosa‘Akami-ohshima’ | 大岛樱系园艺栽培品种Horticultural cultivar of C.speciosa series |
10 | 八重红大岛樱C.speciosa‘Yaebeni-ohshima’ | 大岛樱系园艺栽培品种Horticultural cultivar of C.speciosa series |
11 | 白花真樱C.serrulata‘Multiplex’ | 日本晚樱×樱桃 C.serrulata var. lannesiana×C.pseudocerasus |
12 | 白妙樱C.serrulata‘Sirotae’ | 真樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.serrulata |
13 | 千里香樱C.serrulata‘Senriko’ | 真樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata |
14 | 太白樱C.serrulata‘Taihaku’ | 真樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata |
15 | 雨宿樱C.serrulata‘Amayadori’ | 真樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata |
16 | 大提灯樱C.serrulata‘Ojochin’ | 真樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata |
17 | 兰兰樱C.serrulata‘Ranran’ | 真樱影响的日本晚樱(白兰×雨宿) C.serrulata var.lannesiana influenced by C.serrulata(ハクラン×C.serrulata‘Amayadori’) |
18 | 兼六园菊樱C.serrulata‘Sphaerantha’ | 山樱大山樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii |
19 | 关山樱C.serrulata‘Kanzan’ | 山樱大山樱影响的日本晚樱C.serrulata var.lannesiana influenced by Cserrulata and Csargentii |
20 | 大村樱C.serrulata‘Mirabilis’ | 山樱大山樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.serrulata and C.sargentii |
21 | 红华樱C.serrulata‘Kouka’ | 山樱大山樱影响的日本晚樱(大山樱× 日本晚樱) C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii(C.sargentii×C.serrulata var.lannesiana) |
22 | 手弱女樱C.serrulata‘Taoyame’ | 山樱大山樱影响的日本晚樱(大岛×山樱) C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii(C.speciosa×C.serrulata) |
23 | 梅护寺数珠挂樱C.serrulata‘Juzukakezakura’ | 山樱大山樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii |
24 | 山樱枝垂樱C.serrulata‘Sendai-shidare’ | 山樱大山樱影响的日本晚樱(大岛×未知) C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii(C.speciosa×Unknow) |
25 | 鹎樱C.serrulata‘Longipedunculata’ | 霞樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.verecunda |
26 | 菊垂樱C.serrulata‘Plena-pendula’ | 霞樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.verecunda |
27 | 红玉锦樱C.serrulata‘Beni-tamanishiki’ | 霞樱影响的日本晚樱(八重霞樱×日本晚樱) C.serrulata var. lannesiana influenced by C.verecunda(C.verecunda f.plena×C.serrulata var.lannesiana) |
28 | 松前红绯衣樱C.serrulata‘Matsumae-benihigoromei’ | 高岭樱影响的日本晚樱(高岭樱×日本晚樱) C.serrulata var. lannesiana influenced by C.nipponica(C.nipponica×C.serrulata var.lannesiana) |
29 | 骏河台香樱C.speciosa‘Surugadai-odora’ | 大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa |
30 | 御车返樱C.serrulata‘ Mikurumakaisi’ | 大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa |
31 | 天川樱C.serrulata‘Erecta’ | 大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa |
32 | 一叶樱C.serrulata‘Hisakura’ | 大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa |
33 | 松月樱C.serrulata‘Superba’ | 大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa |
34 | 普贤象樱C.serrulata‘Albo-rosea’ | 大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa |
35 | 御衣黄樱C.serrulata‘Gioiko’ | 大岛樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.speciosa |
36 | 郁金樱C.serrulata‘Grandiflora’ | 大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa |
37 | 日暮樱C.serrulata‘Amabilis’ | 大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa |
38 | 苔清水樱C.serrulata‘Kokeshimidsu’ | 大岛樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.speciosa |
39 | 福禄寿樱C.serrulata‘Contorta’ | 福禄寿系的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata‘Contorta’ systems |
40 | 花笠樱C.serrulata‘Hanagasa’ | 福禄寿系的日本晚樱(“福禄寿”的实生苗) C.serrulata var.lannesiana influenced by C.serrulata‘Contorta’series(Seedling of C.serrulata‘Contorta’) |
41 | 白雪樱C.serrulata‘Sirayuki’ | 白雪系的日本晚樱C.serrulata var.lannesiana influenced by C.serrulata‘Sirayuki’series |
42 | 红笠樱C.serrulata‘Benigasa’ | 江户系园艺栽培品种(丝括×日本晚樱) Horticultural cultivar of C.serrulata‘Nobilis’ series(C.serrulata‘Fasciculata’×C.serrulata var.lannesiana) |
43 | 杨贵妃樱C.serrulata‘Mollis’ | 江户系的日本晚樱C.serrulata var.lannesiana influenced by C.serrulata‘Nobilis’ series |
44 | 红手毬樱 C.serrulata‘Benitemari’ | 江户系园艺栽培品种 Horticultural cultivar of C.serrulata‘Nobilis’ series |
45 | 东锦樱C.serrulata‘Azuma-nishiki’ | 江户系的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata‘Nobilis’ systems series |
46 | 红时雨樱C.serrulata‘Beni-shigure’ | 江户系的日本晚樱(东锦的实生苗) C.serrulata var. lannesiana influenced by C.serrulata‘Nobilis’ series(Seedling of C.serrulata‘Azuma-nishiki’) |
47 | 高砂樱C.× sieboldii‘Caepitosa’ | 高砂系的日本晚樱(丁字樱×日本晚樱) C.serrulata var.lannesiana influenced by C.×sieboldii‘Caepitosa’series(C.apetala×C.serrulata var.lannesiana) |
48 | 红丰C.serrulata‘Beni-yutaka’ | 松前早咲×龙云院红八重C.serrulata‘Matsumae-hayazaki’ ×C.serrulata‘Ryuun-in-beniyae’ |
49 | 旭山.朝日山樱C.serrulata‘Asahiyama’ | 系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana |
50 | 麒麟樱C.serrulata‘Kirin’ | 系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana |
51 | 朱雀樱C.serrulata‘Shujaku’ | 系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana |
52 | 白山2号樱Cerasus‘Hakusan-nigo’ | 系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana |
53 | 弘前三段咲樱Cerasus‘Hirosaki-sandanzaki’ | 系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana |
54 | 蝴蝶樱C.serrulata‘Kocho’ | 系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana |
江户彼岸群Cerasus subhirtella group | ||
55 | 江户彼岸樱C.subhirtella | 日本原生种Japanese native species |
56 | 八重红枝垂樱C.subhirtella‘Plena-rosea’ | 彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series |
57 | 十月樱C.subhirtella‘Autumnalis’ | (江户彼岸×豆樱)×小彼岸(C.subhirtella×C.incisa)×C.subhirtella‘Subhirtella’ |
58 | 神代曙樱C.yedoensis‘Jindai-akebono’ | 江户彼岸×未知C.subhirtella×Unknown |
59 | 雨晴垂枝樱C.subhirtella‘Ujou-shidare’ | 彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series |
60 | 小松乙女樱C.subhirtella‘Komatsu-otome’ | 彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series |
61 | 越之彼岸樱C.subhirtella‘Koshinensis’ | 江户彼岸×大山樱C.subhirtella×C.sargentii |
62 | 十月红樱C.subhirtella‘Autumnalis Rosea’ | 彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series |
63 | 澳博拉大叶早樱C.subhirtella‘Ombrella’ | 彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series |
64 | 思川樱C.subhirtella‘Omoigawa’ | 十月樱×未知C.subhirtella‘Autumnalis’×Unknown |
65 | 红枝垂樱C.subhirtella‘Pendula Rosea’ | 彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series |
66 | 染井吉野樱C.yedoensis | 江户彼岸×大岛樱C.subhirtella×C.speciosa |
67 | 嘉奖樱C.subhirtella‘Accolade’ | 大山樱×江户彼岸C.sargentii×C.subhirtella |
68 | 小彼岸樱C.subhirtella‘Subhirtella’ | 豆樱×江户彼岸C.incisa×C.subhirtella |
69 | 御帝吉野樱C.yedoensis‘Mikado-yoshino’ | 大岛樱×江户彼岸C.speciosa×C.subhirtella |
70 | 潘多拉樱Cerasus‘Pandora’ | 大叶早樱某品种×染井吉野C.subhirtella‘Ascendens Rosea’×C.yedoensis |
71 | 咲耶姬樱C.yedoensis‘Sakuyahime’ | 染井吉野实生苗Seedling of C.yedoensis |
72 | 阳春樱Cerasus‘Yoshun’ | 亲本不详Unknown parent |
寒绯樱群Cerasus campanulata group | ||
73 | 寒绯樱C.campanulata | 中国台湾原生种Taiwan of China native species |
74 | 琉球寒绯樱C.campanulata‘Ryukyu-hizakura’ | 原产我国台湾及琉球群岛Originated in Taiwan and Ryukyu Islands of China |
75 | 阳光樱C.campanulata‘Youkou’ | 天城吉野×寒绯樱C.yedoensis‘Amagi-yoshino’×C.campanulata |
76 | 寒樱C.campanulata‘Kanzakura’ | 寒绯樱×山樱C.campanulata×C.serrulata |
77 | 大寒樱C.campanulata‘Oh-kanzakura’ | 寒绯樱×大岛C.campanulata×C.speciosa |
78 | 修善寺寒樱C.campanulata‘Rubescens’ | 寒绯樱×大岛C.campanulata×C.speciosa |
79 | 才力樱C.campanulata‘Okame’ | 寒绯樱×豆樱C.campanulata×C.incisa |
80 | 初美人樱C.campanulata‘Introrsa’ | 寒绯樱×樱桃C.campanulata×C.pseudocerasus |
81 | 河津樱C.campanulata‘Kawazu-zakura’ | 寒绯樱×大岛樱C.campanulata×C.speciosa |
82 | 横滨绯樱C.campanulata‘Kenrokuen-kumagai’ | 兼六园熊谷×寒绯樱C.jamasakura‘Kenrokuen-kumagai’×C.campanulata |
83 | 大渔樱C.campanulata‘Tairyo-zakura’ | 早咲大岛樱×寒樱C.lannesiana var.speciosa×C.campanulata‘Kanzakura’ |
84 | 粉巨人樱Cerasus‘Pin giant’ | 亲本不详Unknown parent |
豆樱群Cerasus incisa group | ||
85 | 三岛富士樱C.incisa | 日本原生种Japanese native species |
86 | 阿丽亚娜富士樱C.incisa‘Ariane’ | 豆樱类品种Variety of C.incisa |
87 | 维多利富士樱C.incisa‘Vitroeli’ | 豆樱类品种Variety of C.incisa |
88 | 冬樱C.incisa‘Parvifolia’ | 豆樱×大岛樱C.incisa×C.speciosa |
表4
变异位点分析
序列名称Sequence name | 变异位点Variable | |
---|---|---|
简约信息位点Parsim-info | 单突变位点Singleton | |
ITS | A-C(41,190,414,509),C-A(44,112,573),C-T(63,145,163,184,205),G-T(91,94,445),A-G(96),C-G(432),T-G(438),G-A(567),A-T(580) | C-T(95,111,372,469,534,590),G-A(317),G-C(502,559),A-C(504,512),T-G(502) |
trnH-psbA | C-A(41),G-T(281) | A-T(126,130),T-A(133),C-A(300) |
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