超声辅助酸水解提取山里红叶中牡荆素的工艺优化

doi:10.7525/j.issn.1673-5102.2020.01.020

植物研究 ›› 2020, Vol. 40 ›› Issue (1): 148-152.doi: 10.7525/j.issn.1673-5102.2020.01.020

超声辅助酸水解提取山里红叶中牡荆素的工艺优化

邢文淼, 陈民, 冯昌寅, 孟宪明, 田梦飞, 牟璠松, 罗猛

东北林业大学化学化工与资源利用学院, 哈尔滨 150040

收稿日期:2019-06-16 出版日期:2020-01-05 发布日期:2020-02-21
通讯作者: 罗猛 E-mail:wslm_5555@nefu.edu.cn
作者简介:邢文淼(1992-),女,硕士,主要从事植物活性成分研究。
基金资助:
国家重点研发计划项目（2017YFD0600605）；黑龙江省博士后科研启动金（LBH-Q13003）；中央高校基本科研业务费专项资金项目（2572014CA08）

Optimization of Ultrasound Assisted Acid Hydrolysis Process for Extracting Vitexin from Crataegus pinnatifida Bge. var. major N.E.Br. Leaf

XING Wen-Miao, CHEN Min, FENG Chang-Yin, MENG Xian-Ming, TIAN Meng-Fei, MU Fan-Song, LUO Meng

College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040

Received:2019-06-16 Online:2020-01-05 Published:2020-02-21
Supported by:
National Key Research & Development Program of China(2017YFD0600605);Heilongjiang Postdoctoral Funds for Scientific Research Initiation(LBH-Q13003);Fundamental Research Funds for the Central Univ(2572014CA08)

摘要/Abstract

摘要： 采用正交法优化山里红叶中牡荆素的超声辅助提取工艺。通过盐酸浓度、提取时间、固液比、超声功率、超声温度、乙醇浓度6种影响因素的单因素实验，研究它们与牡荆素提取得率之间的规律趋势；应用正交设计优化试验，研究盐酸浓度、提取时间、固液比和超声功率对山里红叶提取牡荆素得率影响大小，并确定最佳提取工艺。研究结果表明：当盐酸浓度为2 mol·L^-1、提取时间为40 min，固液比为1：20、超声功率为500 W、超声温度为50℃、乙醇浓度为50%时，效果最佳，得率为2.603 mg·g^-1；盐酸浓度具有较大显著性，且各因素影响顺序为：盐酸浓度 > 超声功率> > 提取时间 > 固液比。

关键词: 山里红叶, 牡荆素, 提取工艺, 超声辅助, 正交试验

Abstract: Ultrasound-assisted extraction of vitexin from Crataegus pinnatifida Bge. var. major N.E.Br. leaf was optimized by orthogonal design. Through the single factor experiments of six factors the HCl content, extraction time, solid-liquid ratio, ultrasonic power, ultrasonic temperature and ethanol concentration, the regularity trend between them and the vitexin extraction yield was studied. The optimization experiment with orthogonal design was conduted to study the effects of HCl content, extraction time, solid-liquid ratio and ultrasonic power on the extraction yield of C.pinnatifida Bge. var. major N.E.Br. and the optimal extraction process were determined. When the HCl content was 2 mol·L^-1, the extraction time was 40 min, the solid-liquid ratio was 1:20, the ultrasonic power was 500 W, the ultrasonic temperature was 50℃, and the ethanol concentration was 50%, the best result was obtained and the yield was 2.603 mg·g^-1. The HCl content was significant, and the order of influence of each factor was:HCl concentration > ultrasonic power > extraction time > solid-liquid ratio.

Key words: Crataegus pinnatifida Bge. var.major N.E.Br. Leaf, vitexin, extraction technology, ultrasound-assisted, orthogonal test

中图分类号:

Q949.95

邢文淼, 陈民, 冯昌寅, 孟宪明, 田梦飞, 牟璠松, 罗猛. 超声辅助酸水解提取山里红叶中牡荆素的工艺优化[J]. 植物研究, 2020, 40(1): 148-152.

XING Wen-Miao, CHEN Min, FENG Chang-Yin, MENG Xian-Ming, TIAN Meng-Fei, MU Fan-Song, LUO Meng. Optimization of Ultrasound Assisted Acid Hydrolysis Process for Extracting Vitexin from Crataegus pinnatifida Bge. var. major N.E.Br. Leaf[J]. Bulletin of Botanical Research, 2020, 40(1): 148-152.

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超声辅助酸水解提取山里红叶中牡荆素的工艺优化

Optimization of Ultrasound Assisted Acid Hydrolysis Process for Extracting Vitexin from Crataegus pinnatifida Bge. var. major N.E.Br. Leaf

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