关键词: 翅果油树, 叶片, 总黄酮, 大孔吸附树脂, 分离纯化

Abstract: The content of total flavonoids in Elaeagnus mollis Diels leaves was determined by ultraviolet spectrophotometry,the adsorption capacity and desorption rate of four kinds of macroporous resins(D4020、AB-8、S-8、X-5) were calculated to determine the suitable resin and its optimum process conditions. The results indicated: S-8 resin had the highest adsorption capacity(9.83 mg·g^-1), followed by AB-8(9.38 mg·g^-1), but the desorption rate of AB-8 resin(88.79%) was better than that of S-8 resin(71.89%).AB-8 resin was the best absorbent for the total flavonoids in E.mollis leaves. The optimum process conditions were: the pH value of extracts was 3.0~4.0, the ratio of absorbing solution to resin was 6∶1, the adsorption flow rate was 2 mL·min^-1, the eluting solution was 60% ethanol, the eluting flow rate was 2 mL·min^-1, the volume of the eluting solution was 3BV.

Key words: Elaeagnus mollis, leaves, total flavonoids, macroreticular absorption resin, separation and purification