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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (4): 613-625.doi: 10.7525/j.issn.1673-5102.2022.04.011

• Genetic and Breeding • Previous Articles     Next Articles

Genome‑wide Identification and Stress Response Analysis of Cyclic Nucleotide-gated Channels(CNGC) Gene Family in Populus trichocarpa

Xueying WANG1, Ruiqi WANG1, Yang ZHANG1, Cong LIU1, Dean XIA1, Zhigang WEI2()   

  1. 1.State Key Laboratory of Forest Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
    2.Saline Soil Research Center,National Forestry and Grassland Administration,Chinese Academy of Forestry,Beijing 100091
  • Received:2021-05-24 Online:2022-07-20 Published:2022-07-15
  • Contact: Zhigang WEI E-mail:zhigangwei1973@163.com
  • About author:WANG Xueying(1996—),male,postgraduate,mainly engaged in forest tree genetics and breeding research.
  • Supported by:
    Central Public-interest Scientific Institution Basal Research Fund(CAFYBB2020ZA005);National Natural Science Foundation of China(31770640)


The plant cyclic nucleotide-gated channels(CNGC) family has a variety of biological functions and plays an important role in the growth and development of plants and the response to stress. In this study, bioinformatics methods and qRT-PCR were used to analyze the basic physicochemical properties, structural characteristics, phylogeny, gene structure and conserved motif, cis-acting elements of the promoter and gene expression patterns of the PtrCNGC gene family members respectively. The results showed that a total of 19 PtrCNGC genes were identified in the whole genome of Populus trichocarpa. PtrCNGC gene family could be divided into four subgroups(Ⅰ, Ⅱ, Ⅲ and Ⅳ), and subgroup Ⅳ could be divided into two subgroups(Ⅳa and Ⅳb). The proteins encoded by the PtrCNGC genes were all basic proteins, and only one member of the PtrCNGC gene family was hydrophobic protein, while all the other members were hydrophilic proteins. A total of 19 PtrCNGCs were distributed unevenly on 11 chromosomes of P. trichocarpa, while the remaining 8 chromosomes had no members distributed. The PtrCNGC gene family contained seven pairs of homologous genes and the Ka/Ks value between them was far less than one. There was little difference in gene structure and protein conserved motif distribution among the family members. Predictive analysis of cis-acting elements in the promoter region of the PtrCNGC gene sequence showed that there were acting elements related to various hormones and stress. The qRT-PCR results showed that the expression of PtrCNGC gene family was specific in different tissues, with higher expression levels in stems and lower expression levels in roots and leaves. Under salt stress and drought stress, most members of the same branch of the PtrCNGC gene family showed similar expression patterns. The results of this study provid a reference for further study on the function of PtrCNGC family in abiotic stress.

Key words: Populus trichocarpa, CNGC gene family, bioinformatic analysis, abiotic stresses

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