Analysis of the Promoter Sequence and Response Characteristics of the BpPIN3 gene in Betula platyphylla

doi:10.7525/j.issn.1673-5102.2022.04.009

Abstract

Abstract:

As an important auxin efflux carrier family in plants， PIN protein family shows extensive physiological effects in plant growth and development. In order to further understand the function of BpPIN3 and explore its response to different hormone signals and abiotic stresses during Betula platyphylla development，the BpPIN3 promoter sequence was analyzed by bioinformatics methods. Tissue expression patterns of roots， stems， leaves and apical buds of annual and biennial B. platyphylla clones were analyzed respectively. The seedlings were used as conduct hormone induction and light stress treatment with 100 μmol·L^-1 auxin^{（IAA）， 100 μmol·L^-1 gibberellin（GA₃）， 200 μmol·L^-1 abscisic acid（ABA） and long light conditions， respectively. RNA was extracted from leaves and roots of B. platyphylla at 0， 2， 4， 8， 16， 24 and 48 h after hormone treatment and at 0， 1， 3， 6， 12， 24， 48 and 72 h after light stress， and qRT-PCR was used to analyze the expression of BpPIN3. The results showed that the BpPIN3 promoter sequence contained different types of auxin response elements such as gibberellin， abscisic acid， and methyl jasmonate， as well as multiple stress-related cis-regulatory elements. BpPIN3 was expressed in many tissues of birch in different growth years， especially in leaves， and the expression of BpPIN3 in the second year was higher than that in the first year. The relative expression of BpPIN3 gene was different in different parts under different treatment conditions. IAA and GA₃ could induce the up-regulation of BpPIN3 expression in leaf. Under ABA treatment， except for 16 and 48 h， the expression pattern of BpPIN3 gene was opposite to that under IAA treatment. IAA， GA₃ and ABA could induce the expression of BpPIN3 in root， but shading stress induced the expression of BpPIN3 gene in leaf， the relative expression of BpPIN3 gene was significantly higher than that of the control（0 h） from 12 h in root， IAA， GA₃ and ABA could induce the expression of BpPIN3 in root， shading stress induced the expression of BpPIN3 gene in leaf； the relative expression of BpPIN3 gene was significantly higher than that of the control （0 h）from 12 h in root. The results suggested that BpPIN3 gene played the important regulatory role in the growth and development of B. platyphylla，and the signal transduction pathways of IAA， GA₃and ABA and plant light response.}

Key words: Betula platyphylla, BpPIN3, plant hormones, light response

CLC Number:

S792.153

Kun CHEN, Gonggui FANG, Huaizhi MU, Jing JIANG. Analysis of the Promoter Sequence and Response Characteristics of the BpPIN3 gene in Betula platyphylla[J]. Bulletin of Botanical Research, 2022, 42(4): 592-601.

Figures/Tables 5

Table 1

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顺式作用元件 Cis-acting element	核心序列 Core sequence	数量 Number	功能 Function
ABRE	ACGTG	7	ABA响应元件 Responsive to ABA
ARE	AAACCA	1	厌氧诱导的必须调控元件 Regulatory element essential for anaerobic induction
BOX4	ATTAAT	1	光响应元件 Light responsive element
CAAT-box	CAAT	35	顺式作用元件 cis-acting element
CCAAT-box	CAACGG	1	MYBHv1结合位点 MYBHv1 binding site
CGTCA-motif	CGTCA	1	MEJA顺式作用调节元件 MEJA cis-acting regulatory element
G-box	CACGTT	7	光响应元件 Light responsive element
GARE-box	TCTGTTG	2	赤霉素反应元件 Gibberellin-responsive element
GT1-motif	GGTTAA	2	光响应元件 Light responsive element
HD-Zip 3	GTAAT（G/C）ATTAC	1	蛋白结合位点 Protein binding site
LTR	CCGAAA	2	低温响应顺式作用元件 Low-temperature responsiveness cis-acting element
MBS	CAACTG	1	MYB结合位点参与干旱诱导MYB binding site involved in drought-inducibility
P-box	CCTTTG	1	赤霉素反应元件 Gibberellin-responsive element
TATA-box	TATA	58	转录起始-30核心启动子元件 Core promoter element around-30 of transcription start
TCA-element	CCATCTTTT	1	水杨酸反应元件 Salicylic-responsive element
TCT-motif	TGACG	1	光响应元件Light responsive element

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