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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (6): 913-922.doi: 10.7525/j.issn.1673-5102.2020.06.014

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Cloning,Structure and Function Analysis of MiMYB2 Gene from Macadamia integrifolia

Wen-Lin WANG1, Hai-Sheng CHEN1(), Shu-Fang ZHENG1, Song-Le FAN2,3, Li-Feng WANG3, Qiu-Jin TAN1, Zhen-Shi QIN1, Xi-Yun HUANG1, Peng HE1, Xiu-hua TANG1, Peng XU1   

  1. 1.Guangxi South Subtropical Agricultural Research Institute,Longzhou 532400
    2.Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource,College of Tropical Crop,Hainan University,Haikou 570228
    3.Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,P. R. China;State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops;Danzhou Investigation & Experiment Station of Tropical Crops,Ministry of Agriculture and Rural Affairs,P. R. China; Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101
  • Received:2019-12-05 Online:2020-11-20 Published:2020-11-04
  • Contact: Hai-Sheng CHEN E-mail:chenhs6179@163.com
  • About author:WANG Wen-Lin(1980—),senior agronomist, mainly engaged in the genetic and breeding research of tropical fruit trees.
  • Supported by:
    Guangxi Science and Technology Base and Talent Special Project(Guike AD18281087);Chongzuo Science and Technology Plan Project(Chongkegong(2019027);2019039;Guangxi Academy of Agricultural Sciences Science and Technology Development Fund (Guinongke 2019ZX2015,2019ZX17,2020YM148);Basic Scientific Research Fund of Guangxi Academy of Agricultural Sciences(Guinongke 2020YM46,2020YM141)


In order to study the mechanism of R2R3-MYB transcription factor gene family member in growth,development and yield formation in Macadamia integrifolia,the MiMYB2 gene was cloned from the leaves of M.integrifolia “GUIRE1” cultivar by PCR and its structure and function were analyzed by bioinformatics method. The cloned cDNA sequence of MiMYB2(NCBI accession number:MN254976) gene is 1 210 bp in length,has 1002 bp open reading frame(ORF),encoding 333 amino acids. MiMYB2,an unstable hydrophilic protein that has no transmembrane structure,no signal peptide and localizes in the nucleus,has two SANT conserved domains belonging to the R2R3-MYB family. BLAST analysis showed that the MiMYB2 share high amino acid sequence identity with Lotus NnMYB3-like protein. Phylogenetic analysis clustered MiMYB2 and AtMYB17,AtMYB106 and AtMYB16 into S9 subfamily. The transcriptome data were used to analyze the expression patterns of MiMYB2 gene in branch,flower and leave of M.integrifolia “GUIRE1” and “695” cultivar. Results indicated that MiMYB2 has the lowest expression in the flower of “GUIRE1”,and the expression level of “695” is the highest. It is speculated that MiMYB2 is closely related to M.integrifolia growth and development. This study would provide a theoretical reference for clarification the role MiMYB2 in growth,development and yield formation mechanism of M.integrifolia.

Key words: Macadamia integrifolia, MiMYB2, gene cloning, bioinformatics analysis

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