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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (4): 602-612.doi: 10.7525/j.issn.1673-5102.2020.04.015

• Research Report • Previous Articles    

Cloning and Bioinformatics Analysis of LoERF017 Gene from Larix kaempferi

MA Miao-Miao, LI Cheng-Hao, LIU Xiao, ZHANG Xin, YANG Jing-Li   

  1. State Key Laboratory of Forest Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-11-18 Published:2020-06-12
  • Supported by:
    Genetically Modified Organisms Breeding Major Project of China(2018ZX08020-003)

Abstract: The LoERF017 gene cloned from Larix kaempferi was sequenced and found to be 624 bp in length, encoding a total of 207 amino acids. The protein encoded by LoERF017 is predicted to include only one AP2 domain, and its secondary structure includes α-helix (30.43%), random coil (54.11%), extended strand 12.56%), and β-sheet (2.9%). Subcellular localization of the gene showed that LoERF017 was located in the nucleus. The results of qRT-PCR showed that the expression level of LoERF017 gene in L.kaempferi had certain tissue specificity. The expression of this gene in root was the highest, the expression in stem was the lowest, and the expression in leaves was middle. Under drought stress, the expression of LoERF017 gene increased in the root, but decreased after rehydration. It was preliminarily predicted that this gene is mainly expressed and functions in root of L.kaempferi, and it was speculated that LoERF017 might be related to drought resistance.

Key words: Larix kaempferi, LoERF017, bioinformatics, abiotic stress

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