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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (1): 148-152.doi: 10.7525/j.issn.1673-5102.2020.01.020

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Optimization of Ultrasound Assisted Acid Hydrolysis Process for Extracting Vitexin from Crataegus pinnatifida Bge. var. major N.E.Br. Leaf

XING Wen-Miao, CHEN Min, FENG Chang-Yin, MENG Xian-Ming, TIAN Meng-Fei, MU Fan-Song, LUO Meng   

  1. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040
  • Received:2019-06-16 Online:2020-01-05 Published:2020-02-21
  • Supported by:
    National Key Research & Development Program of China(2017YFD0600605);Heilongjiang Postdoctoral Funds for Scientific Research Initiation(LBH-Q13003);Fundamental Research Funds for the Central Univ(2572014CA08)

Abstract: Ultrasound-assisted extraction of vitexin from Crataegus pinnatifida Bge. var. major N.E.Br. leaf was optimized by orthogonal design. Through the single factor experiments of six factors the HCl content, extraction time, solid-liquid ratio, ultrasonic power, ultrasonic temperature and ethanol concentration, the regularity trend between them and the vitexin extraction yield was studied. The optimization experiment with orthogonal design was conduted to study the effects of HCl content, extraction time, solid-liquid ratio and ultrasonic power on the extraction yield of C.pinnatifida Bge. var. major N.E.Br. and the optimal extraction process were determined. When the HCl content was 2 mol·L-1, the extraction time was 40 min, the solid-liquid ratio was 1:20, the ultrasonic power was 500 W, the ultrasonic temperature was 50℃, and the ethanol concentration was 50%, the best result was obtained and the yield was 2.603 mg·g-1. The HCl content was significant, and the order of influence of each factor was:HCl concentration > ultrasonic power > extraction time > solid-liquid ratio.

Key words: Crataegus pinnatifida Bge. var.major N.E.Br. Leaf, vitexin, extraction technology, ultrasound-assisted, orthogonal test

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