Rapid Propagation of Virus-free “Dan Mei 1” Strawberry Using Tissue Culture Technology

doi:10.7525/j.issn.1673-5102.2020.01.021

Abstract

Abstract: The superior "Dan Mei 1" strawberry variety that is suitable for growing in Northeast China were selected as the research object, and the tissue culture technology for detoxification and rapid propagation of strawberry was carried out systematically. The effects of different plant hormones on adventitious bud induction, proliferation and rootage were investigated using the stem tip of "Dan Mei 1" strawberry as explants. Also, the effects of transplanting medium on the domestication survival of strawberry plantlets were investigated. The results showed that MS with 1 mg·L^-1 6-BA+0.1 mg·L^-1 2,4-D was suitable for adventitious bud induction. The best medium for adventitious bud proliferation was MS with 1 mg·L^-1 6-BA+0.3 mg·L^-1 NAA. And, MS with 0.4 mg·L^-1 IBA was the best medium for adventitious bud rootage. Perlite:vermiculite:nursery soil with the ratio of 1:2:1 was the best transplanting medium for the domestication of strawberry plantlets. Overall, this study provided a theoretical foundation for the large-scale propagation of the virus-free "Dan Mei 1" strawberry variety.

Key words: “Dan Mei 1” strawberry, stem tip differentiation, rapid proliferation, virus-free plantlets

CLC Number:

S668.4

WANG Xin, GAI Qing-Yan, JIAO Jiao, FU Yu-Jie, LIU Jing, WANG Zi-Ying. Rapid Propagation of Virus-free “Dan Mei 1” Strawberry Using Tissue Culture Technology[J]. Bulletin of Botanical Research, 2020, 40(1): 153-160.

References

[1] 邵婉璐,李月灵,高松,等.光照强度对成熟红颜草莓果实着色和花青素生物合成的影响及可能的分子机制[J].植物研究,2018,38(5):661-668.Shao W L,Li Y L,Gao S,et al.Effects of light intensity on the fruit coloration and anthocyanian biosynthesis in Fragaria×ananassa Duch."Benihoppe" and the possible molecular mechanism[J].Bulletin of Botanical Research,2018,38(5):661-668.
[2] Bullock J M,Moy I L,Coulson S J,et al.Habitat-specific dispersal:environmental effects on the mechanisms and patterns of seed movement in a grassland herb Rhinanthus minor[J].Ecography,2003,26(5):692-704.
[3] 黄文江,潘超,阚显照,等."红丰"草莓无菌系及叶盘再生系统的建立[J].西南农业学报,2010,23(5):1640-1643.Huang W J,Pan C,Kan X Z,et al.Tissue culture and establishment of regeneration system from leaves of fragaria ananassa duehesene[J].Southwest China Journal of Agricultural Sciences,2010,23(5):1640-1643.
[4] 张连忠,李文金,蔚承祥,等.四季草莓"赛娃"的组织培养与快速繁殖[J].植物生理学报,2002,38(1):39.Zhang L Z,Li W J,Wei C X,et al.Tissue culture and rapid propagation of Fragaria vesca cv.Selva[J].Plant Physiology Journal,2002,38(1):39.
[5] 卢艳丽,周洪友,张笑宇.马铃薯茎尖脱毒方法优化及病毒检测[J].作物杂志,2017(1):161-167.Lu Y L,Zhou H Y,Zhang X Y.Optimization of virus-free of shoot tip and virus detection of test-tube plantlet of potato[J].Crops,2017(1):161-167.
[6] 王鹏,纪瑞瑞,孔祥远,等.高效甘薯脱毒苗生产及驯化移栽[J].植物生理学报,2015,51(4):455-458.Wang P,Ji R R,Kong X Y,et al.Production,acclimatization and transplantation of virus-free plantlets of sweet potato[J].Plant Physiology Journal,2015,51(4):455-458.
[7] 侯少霞,赵永秀,李佳雯,等.生长调节剂对香石竹离体快繁的影响[J].分子植物育种,2018,16(3):938-942.Hou S X,Zhao Y X,Li J W,et al.Effects of different growth regulators on the rapid propagation in vitro of Dianthus caryophyllus[J].Molecular Plant Breeding,2018,16(3):938-942.
[8] 赵霜.菊花病毒脱除与检测技术研究[D].北京:北京林业大学,2013.Zhao S.Study on virus-elimination and virus-detection technique of chrysanthemum[D].Beijing:Beijing Forestry University,2013.
[9] 韩柏明,李贺,高秀岩,等.组培条件下脱毒与带毒丰香草莓植株表型的比较[J].沈阳农业大学学报,2009,40(4):400-403.Han B M,Li H,Gao X Y,et al.Effects of tissue culture and virus elimination on the phenotype of Fragaria×ananassa cv.toyonoka[J].Journal of Shenyang Agricultural University,2009,40(4):400-403.
[10] 李秀华,杜贞,武银玉,等.大花萱草组培快繁体系的研究[J].植物研究,2009,29(6):757-762.Li X H,Du Z,Wu Y Y,et al.Research on tissue culture regeneration of Hemerocallis middendorfii[J].Bulletin of Botanical Research,2009,29(6):757-762.
[11] 刘霞,唐铭,许永华,等.茖葱不定芽分化的再生体系[J].吉林农业大学学报,2015,37(4):434-439.Liu X,Tang M,Xu Y H,et al.Research on differentiation and regeneration system of adventitious buds of Allium victorialis L.[J].Journal of Jilin Agricultural University,2015,37(4):434-439.
[12] 王忠武,建德锋.八仙花茎尖离体培养技术研究[J].北方园艺,2012(9):129-130.Wang Z W,Jian D F.Study on the shoot-tip culture of Hydrangea macrophylla in vitro[J].Northern Horticulture,2012(9):129-130.
[13] 袁维风,徐德聪,钱玉梅,等.草莓"达斯莱克特"的离体再生[J].基因组学与应用生物学,2009,28(4):770-774.Yuan W F,Xu D C,Qian Y M,et al.Regeneration of strawberry "Dasrlect" in vitro[J].Genomics and Applied Biology,2009,28(4):770-774.
[14] 梁丽琨,林荣双,由翠荣,等.不同激素对花生离体分化的影响[J].植物研究,2004,24(2):187-191.Liang L K,Lin R S,You C R,et al.Effects of different plant hormones In-vitro differentiation of peanut[J].Bulletin of Botanical Research,2004,24(2):187-191.
[15] 崔秋华,孙永玉,李昆,等.不同因子对齿瓣石斛增殖系数和平均苗高的影响[J].中南林业科技大学学报,2012,32(4):200-203.Cui Q H,Sun Y Y,Li K,et al.Effects of different factors on proliferation coefficient of Dendrobium devonianum and average seedling height[J].Journal of Central South University of Forestry & Technology,2012,32(4):200-203.
[16] 李志强,王晶,丁国亮,等.草莓热处理结合茎尖脱毒技术研究[J].北方园艺,2012(5):125-127.Li Z Q,Wang J,Ding G L,et al.Study on heat treatment and shoot tip virus elimination technique of strawberry[J].Northern Horticulture,2012(5):125-127.
[17] 陈林晶,张熹涛,曹玉凤,等.丽格海棠组织培养技术的研究[J].北方园艺,2012(22):122-124.Chen L J,Zhang X T,Cao Y F,et al.Study on tissue culture technology of Rieger begonia[J].Northern Horticulture,2012(22):122-124.
[18] 陈菊,陈国慧.6-BA与NAA浓度配比对何首乌不定芽增殖的影响[J].中国农学通报,2006,22(11):173-173.Chen J,Chen G H.Study of the concentration of 6-BA and NAA in the Rapid Propagation of Polygonum multiflorum Thunb[J].Chinese Agricultural Science Bulletin,2006,22(11):173-173.
[19] 刘玉艳,于凤鸣,于娟.IBA对含笑扦插生根影响初探[J].河北农业大学学报,2003,26(2):25-29.Liu Y Y,Yu F M,Yu J.Primary study on the effects of IBA to the rooting of Michelia figo[J].Journal of Agricultural University of Hebei,2003,26(2):25-29.
[20] 林萍,普晓兰.球花石楠离体培养及快速繁殖研究[J].植物研究,2003,23(3):280-284.Lin P,Pu X L.Rapid propagation of Photinia glomerata in vitro[J].Bulletin of Botanical Research,2003,23(3):280-284.
[21] 石雪晖,陈海霞,蒋辉,等.生长调节剂对草莓试管苗生根及移栽的影响[J].湖南农业大学学报:自然科学版,2003,29(6):509-512.Shi X H,Chen H X,Jiang H,et al.On effects of plant growth regulators on rooting and transplanting of strawberry vitreous[J].Journal of Hunan Agricultural University:National Sciences,2003,29(6):509-512.
[22] 晁慧娟,刘敏,姬谦龙,等. "甜查理"草莓茎尖培养与快速繁殖研究[J].北京农学院学报,2008,23(2):24-27.Chao H J,Liu M,Ji Q L,et al.Stem tip culture and rapid propagation of strawberry "Sweet charlie"[J].Journal of Beijing University of Agriculture,2008,23(2):24-27.