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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (1): 148-155.doi: 10.7525/j.issn.1673-5102.2019.01.018

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Characteristics of Microsatellite in Camellia saluenensis by High-throughput Sequencing

WANG Da-Wei1,2, ZHOU Fan1,2, SHEN Bing-Qi1,2, WANG Lian-Chun1,2   

  1. 1. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224;
    2. Key Laboratory for Forest Resource Conservation and Utilization in the Southwest Mountains of China, Southwest Forestry University, Kunming 650224
  • Received:2018-08-31 Online:2019-01-15 Published:2019-01-31
  • Supported by:
    Open fund of Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province(2017011)

Abstract: Leaves sample of Camellia saluenensis Stapf ex Bean was sequenced with high-throughput sequencing technology(Illumina Hiseq 2000). In total 140 996 transcripts of C.saluenensis were screened using MISA software. The 32 696 SSRs were identified, and the frequency of SSR was 23.2% and mean distribution density was dinucleotide 32.02 kB. The di-nucleotide repeats were the highest, followed by tri-nucleotide and mono-nucleotide repeat types. The tetra-nucleotide, penta-nucleotide and hexa-nucleotide were all less than 1%. Among the mononucleotide repeats, the A/T repeats motifs were the highest frequency(10.92%). AG/CT was the most frequent repeat motifs in di-nucleotide repeats(49.72%), followed by AT/AT and AC/GT. CG/CG were minimum. AAG/CTT repeats motifs was the highest in tri-nucleotide followed by ACC/GGT, ATC/ATG and AGG/CCT, while CCG/GGC, ACT/AGT and ACG/CGT were lowest(<1%). The repeat motifs were very few in tetra-nucleotide, petra-nucleotide and hexa-nucleotide and had no obvious differences. The microsatellite number in C.saluenensis decreased with the increased number of repeats and length of repeats.

Key words: Camellia saluenensis Stapf ex Bean, Transcriptome, microsatellite, high-throughput sequencing

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