Cloning and Expression Analysis of Two PLT Transcription Factors Genes in Fraxinus mandshurica

doi:10.7525/j.issn.1673-5102.2019.01.017

Abstract

Abstract: In order to investigate the function and mechanism of the PLT transcription factor in the root development of Fraxinus mandshurica, we cloned two PLT transcription factors from F.mandshurica, and named them FmPLT2 and FmPLT3 by homologous alignment, encoding 532 and 497 amino acid residues, respectively. By bioinformatics analysis, the relative molecular masses of FmPLT2 and FmPLT3 were 59 and 55 kDa, respectively, and the isoelectric points were 5.98 and 5.79, respectively. They were all hydrophilic unstable proteins, and both contained two AP2 conserved domains. By phylogenetic analysis, FmPLT2 and FmPLT3 had the highest homology with the OePLT2 and OeOLT3 genes of the Oleaceae, respectively. Subcellular localization predictions showed that both FmPLT2 and FmPLT3 proteins were mainly concentrated in the nucleus. The expression of FmPLT2 and FmPLT3 in different tissues and roots of different developmental stages was analyzed by qRT-PCR. The tissue-specific expression of FmPLT2 and FmPLT3 was similar and they all have the highest expression in roots and the lowest expression in leaves. The expression level of FmPLT2 in roots at different developmental stages is extremely significant. Especially, the expression level of FmPLT2 on the 21st day of root development is 32 times as high as that at the 0 day, but the expression of FmPLT3 was not significant, indicating that FmPLT2 not only plays an important role in root development, but also may participate in multiple developmental pathways such as cell proliferation and growth.

Key words: Fraxinus mandshurica, PLT transcription factors, gene cloning, expression analysis, root development

CLC Number:

S722.3+7

DING Yi-Wei, ZHAN Ya-Guang, ZHANG Jia-Wei, HE Li-Ming. Cloning and Expression Analysis of Two PLT Transcription Factors Genes in Fraxinus mandshurica[J]. Bulletin of Botanical Research, 2019, 39(1): 139-147.

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