Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (4): 605-612.doi: 10.7525/j.issn.1673-5102.2016.04.017

Previous Articles     Next Articles

Bioinformatics Analysis of Casbene Synthase Genes in Different Plants

LIU Hong-Wei1, YANG Yan-Fang1, XIONG Wang-Dan2, WU Ping-Zhi2, WU Guo-Jiang2, QIU De-You1   

  1. 1. State Key Laboratory of Forest Tree Genetics and Breeding, the Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091;
    2. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650
  • Received:2016-01-13 Online:2016-07-15 Published:2016-06-15
  • Supported by:
    The National Natural Science Foundation of China(31270705);National non-profit Research Institutions of Chinese Academy of Forestry(RIF2014-01)

Abstract: We used bioinformatics to study 13 casbene synthase (CS; EC full-length gene sequences registered in the GenBank from Euphorbia esula, Jatropha curcas L., Ricinus communis and Triadica sebifera, and predicted the composition of nucleic acid and amino acid sequences, leader peptides, signal peptide, trans-membrane topological structure, hydrophobicity or hydrophilicity, the secondary and tertiary structure as well as the function domains. The 13 genes encoding ORFs were 1647-1845 bp, the molecular weight of the 13 predicted proteins were 63.0-70.8 kD, and the termination codons were TGA or TAA. The theoretical isoelectric points of the 13 proteins were lower than 7.0, which suggested that CS proteins were acidic. Leu was the most contented amino acid. By the homologous alignment of nucleic acid, CS genes were divided into two groups. The prediction of leading peptides showed that at least 6 CSs had leader peptide (chloroplast leader peptide mainly) in common. All 13 CSs had no signal peptide and trans-membrane topological structure in and the peptide chains were hydrophilicity. α-helix was the dominant secondary structure constructional element of the 13 proteins which contained two terpenoid synthases function domains.

Key words: tigliane, casbene synthase, bioinformatics

CLC Number: