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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (1): 77-83.doi: 10.7525/j.issn.1673-5102.2015.01.013

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Cloning and Transient Expression Analysis of Promoter of PnsICE1 from Populus simonii×P.nigra


  1. 1.Forestry Science Research Institute of Heilongjiang Province,Harbin 150081;
    2.Heilongjiang Forestry Academy of Science,Harbin 150081
  • Online:2015-01-15 Published:2015-03-11
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Abstract: ICE1 encodes a MYC-like bHLH transcriptional activator, which could specially bind to active domain of CBF3 promoter and induce transcriptal expression of cold-responsive genes downstream of CBF3, play important roles in stress response. A 1 247 bp sequence of PnsICE1 promoter was cloned from DNA of Populus simonii×P.nigra by PCR amplification method. By sequence analysis, it consisted of a typical core promoter region of eukaryotic, beside the basic elements: TATA-box and CAAT-box, and there were some hormone responsive elements and multiple stress-induced elements of ABRE, DOFCOREZM, MYBCORE, MYCCONSENSUSATHSE and W-box. PnsICE1 promoter may associated with adversity stress and play important roles in response to various stresses in P.simonii×P.nigra. To verify the biology function, recombinant vector was designated as pBI121-ICEPro through replacing CaMV35S promoter in pBI121 by cloned PnsICE1 promoter fragment. In the vector, gus reporter gene was driven by PnsICE1 promoter. GUS histochemical assay in Arabidopsis thaliana after transient infection showed that gus expressed in both flower and root.

Key words: Populus simonii×, P.nigra, PnsICE1 promoter, regulatory element, abiotic stress

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