Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (6): 731-736.doi: 10.7525/j.issn.1673-5102.2012.06.017

Previous Articles     Next Articles

Cloning,Sequence Analysis and Prokaryotic Expression of an Alcohol Acyltransferase(AAT) Gene in Tomato(Solanum lycopersicum)

CAO Ying;HU Shang-Lian;ZHANG Hui-Ying;TANG Xiao-Feng;LIU Yong-Sheng   

  1. 1.Life Science and Engineering College,Southwest University of Science and Technology,Mianyang 621010;2.College of Life Science,Sichuan University,Chengdu 610064;3.Bioengineering College,Chongqing University,Chongqing 400030
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-20 Published:2012-11-20
  • Supported by:

Abstract: Volatile esters are important aromatic compounds accumulated in many ripe fruits. Alcohol acyltransferase (AAT) plays a key role in the formation of volatile esters. In the present study, a full-length cDNA sequence homologous with other plant AAT genes was isolated by RT-PCR from tomato ripe fruit, and named as SlAAT1(GenBank Accession No.JN398667). Results of sequence analyses showed SlAAT1 encodes a 442-amino acid protein, which exhibits conserved features of the BAHD family of acetyl transferase, such as HxxxD and DFGWG motifs. Phylogenetic analysis revealed that SlAAT1 is very closely related to the apple MpAAT1, Clarkia breweri BEBT, and tobacco Hsr201. The recombinant plasmid was transformed into E. coil BL21 (DE3) to express the SlAAT1-His6 protein in the optimized condition (22℃, 0.8 mmol·L-1 IPTG). The purified recombinant SlAAT1 protein was shown to have acetyltransferase activity, suggesting its role in volatile ester formation.

Key words: Alcohol acyltransferase, cloning, prokaryotic expression, ester formation

CLC Number: