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Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (4): 444-451.doi: 10.7525/j.issn.1673-5102.2012.04.012

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Cloning and Sequence Analysis of 1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate Synthase Gene cDNA from Eucommia ulmoides

LIU Pan-Feng;DU Hong-Yan*;DU Lan-Ying;WU YUN Ta-Na;HUANG Hai-Yan   

  1. 1.Non-timber Forestry Research and Development Center,Chinese Academy of Forestry,Zhengzhou 450003;2.Key Laboratory of Nonwood Forest Products of State Forestry Administration,Changsha 410004
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-20 Published:2012-07-20
  • Contact: DU Hong-Yan
  • Supported by:

Abstract: 1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase (HDS) catalyses 2C-methyl-D-erythritol-2,4-cyclodiphosphate into 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate in the penultimate step of 2C-methyl-D-erythritol 4-phosphate (MEP) pathway, which is an alternative plant terpenoids biosynthetic route that has been recently discovered. Homologous HDS cDNA (EuHDS) was isolated from leaves of Eucommia ulmoides by the methods of reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technique. The full-length cDNA of EuHDS was 2 786 bp and encoded 743 amino acids with a predicted molecular mass of 82.25 kD, and a theoretical isoelectric point of 5.89. Two conserved motifs and three absolutely conserved cysteines (residues 644, 647 and 678) were discovered in the deduced coding sequence. Phylogenetic analysis revealed that EuDXR was more similar to Vitis vinifera(evolutionary distance, 0.049) than that of other species, followed by Solanum lycopersicum(0.052) and Hevea brasiliensis(0.052).

Key words: Eucommia ulmoides, HDS, gene, sequence analysis

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