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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (2): 242-244.doi: 10.7525/j.issn.1673-5102.2009.02.020

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A Method for Lower Epidermis Micromorphology Research of Fresh Leaf of Gramineae

YANG Hu-Biao;LI Xiao-Xia;LUO Li-Juan*   

  1. (College of Horticulture & Garden architecture,University of Hainan,Danzhou 571737)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
  • Contact: LUO Li-Juan*
  • Supported by:

Abstract: The aim of this study was to introduce an efficiect and fast method for observation the lower epidermis of fresh leaf of Gramineae, it also can be used for observation the stomata of Monocotyledon whatever fresh leaf or have been fixed in FAA solution. This method can avoid crispation when epidermis dehydration in 100% ethanol and clearing in Xylene to keep the full framework for accurate and efficient observation of microstructure. Furthermore, this method exhibits higher reliability and stability for micromorphology research of leaf epidermis of Gramineae and Monocotyledon.

Key words: Gramineae, fresh leaf, lower epidermis, method

CLC Number: