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植物研究 ›› 2010, Vol. 30 ›› Issue (3): 373-378.doi: 10.7525/j.issn.1673-5102.2010.03.022

• 论文 • 上一篇    下一篇

翅果油树叶总黄酮分离纯化工艺的研究

睢玉祥;张家铭;穆楠;杜贞;闫桂琴*   

  1. 山西师范大学生命科学学院生物多样性研究所,临汾 041004
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
  • 通讯作者: 闫桂琴
  • 基金资助:
     

Separation and Purification Process of Total Flavonoids from Elaeagnus mollis Leaves

SUI Yu-Xiang;ZHANG Jia-Ming;MU Nan;DU Zhen;YAN Gui-Qin*   

  1. School of Life Science,Institue of Biodiversity,Shanxi Normal University,Linfen 041004
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Contact: YAN Gui-Qin
  • Supported by:
     

摘要:

用紫外分光光度法测定翅果油树(Elaeagnus mollis Diels)叶总黄酮的含量,计算D4020、AB-8、S-8、X-5四种树脂的饱和吸附量和解吸率,比较分析各树脂对翅果油树叶总黄酮的分离纯化效果,确定最佳树脂及其最佳工艺条件。结果表明:S-8对翅果油树叶片总黄酮有高的吸附量(9.83 mg·g-1),AB8次之(9.38 mg·g-1),但解吸率AB-8(88.79%)高于S-8(71.89%)。确定所选树脂中AB-8为翅果油树叶总黄酮的最佳吸附剂,最佳工艺条件为:吸附液pH值3.0~4.0、上样量与树脂量之比6∶1、吸附流速2 mL·min-1、洗脱液为60%乙醇、洗脱流速2 mL·min-1、洗脱剂用量3BV。

关键词: 翅果油树, 叶片, 总黄酮, 大孔吸附树脂, 分离纯化

Abstract: The content of total flavonoids in Elaeagnus mollis Diels leaves was determined by ultraviolet spectrophotometry,the adsorption capacity and desorption rate of four kinds of macroporous resins(D4020、AB-8、S-8、X-5) were calculated to determine the suitable resin and its optimum process conditions. The results indicated: S-8 resin had the highest adsorption capacity(9.83 mg·g-1), followed by AB-8(9.38 mg·g-1), but the desorption rate of AB-8 resin(88.79%) was better than that of S-8 resin(71.89%).AB-8 resin was the best absorbent for the total flavonoids in E.mollis leaves. The optimum process conditions were: the pH value of extracts was 3.0~4.0, the ratio of absorbing solution to resin was 6∶1, the adsorption flow rate was 2 mL·min-1, the eluting solution was 60% ethanol, the eluting flow rate was 2 mL·min-1, the volume of the eluting solution was 3BV.

Key words: Elaeagnus mollis, leaves, total flavonoids, macroreticular absorption resin, separation and purification

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