Abstract: Effects of different factors on regeneration of leaves and stems were studied by using Dioscorea bulbifera L. as test material to optimize the regeneration system of leaves and stems of D. bulbifera L. virus-free plantlets. The results showed: The callus induction medium of D. bulbifera L. leaves and stems was MS+KT 2 mg·L^-1+2,4-D 2 mg·L^-1; The best sucrose concentration of callus induction of D. bulbifera L. leaves and stems was 30 g·L^-1 and 50 g·L^-1 respectively; Calli of D. bulbifera L. leaves and stems were induced easily in dark; The differentiation medium of D. bulbifera L. leaf and stem calli was MS+KT 4 mg·L^-1+NAA 0.1 mg·L^-1; The calli of D. bulbifera L. leaves and stems were differentiated easily after subcultured for two times; The best rooting medium of D. bulbifera L. adventitious buds was 1/2MS+IBA 0.1 mg·L^-1+NAA 0.5 mg·L^-1+PP₃₃₃ 1 mg·L^-1. The regeneration system from leaves and stems of D. bulbifera L. virus-free plantlets was established successfully for the first time, which providing a technological basis for factory production of D. bulbifera L. virus-free plantlets.

Key words: Dioscorea bulbifera L., virus-free plantlets, leaf, stem, regeneration system