Meristematic Nodule Induction and Adventitious Shoot Differentiation in Distant Hybrid Tree Peony

doi:10.7525/j.issn.1673-5102.2024.05.007

Abstract

Abstract:

To explore a simple and suitable method for inducing meristematic nodules in multiple cultivars， the petioles of inter-subsectional and inter-sectional distant hybrid tree peony were used as explants， and the conditions for the differentiation of meristematic nodules into adventitious shoot in some cultivars were also identified. The results showed that meristematic nodules were induced by one step in 10 cultivars with 52.08%- 83.33% induction rates， the thin layers of petioles of 11 cultivars were continuously cultured on SH medium containing 0.5 mg·L^-1 NAA+1.0 mg·L^-1 CPPU+0.5（or 0） mg·L^-1 TDZ for 60-100 d in the dark， and meristematic nodules induced by one step were generated indirectly from the callus. Adventitious shoot differentiation rate of three cultivars was 6.67%-33.33% when meristem nodules were added to the modified WPM medium containing 0.5（or 1） mg·L^-1 CPPU+0.5 mg·L^-1 TDZ under light condition and sub-cultured every 15 days. Lower concentrations of CPPU and TDZ together promoted adventitious shoot differentiation， but high concentrations of CPPU alone were not conductive to adventitious shoot differentiation. The general medium for one-step induction of meristematic nodules was screened， and adventitious shoot differentiation was achieved in some cultivars， which provided an important reference for the establishment of plant regeneration system for distant hybrid tree peonies.

Key words: Paeonia × lemoinei, intersectional hybrids of Paeonia, meristematic nodule, adventitious shoot

CLC Number:

S685.11

Yu XIE, Wanting LIU, Jun CUI, Fangyun CHENG, Yuan ZHONG. Meristematic Nodule Induction and Adventitious Shoot Differentiation in Distant Hybrid Tree Peony[J]. Bulletin of Botanical Research, 2024, 44(5): 702-710.

Figures/Tables 7

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编号 No.	品种 Variety	分生结节诱导率 Meristematic nodules induction rate/%
编号 No.	品种 Variety	60 d	75 d	100 d
1	‘Golden Isles’	13.54±2.95a	5.20±1.47b	14.58±1.47a
2	‘Renown’	0a	1.04±1.47b	3.13±0a
3	‘High Noon’	25.00±2.55b	26.04±6.42b	83.33±5.31a
4	‘07-LB-19’	42.71±5.31b	43.75±8.84b	60.42±2.95a
5	‘Oukan’	25.00±5.10a	22.92±8.96a	22.92±1.47a
6	‘Golden Era’	76.04±1.47a	61.46±3.90b	67.71±6.42ab
7	‘Alice Harding’	0b	0b	27.08±2.95a
8	‘Souvenir de Maxime Cornu’	4.17±2.95b	25.00±5.10a	0b
9	‘Lemon Dream’	0b	20.83±2.95a	7.29±1.47b
10	‘Bartzella’	23.96±7.80b	36.46±5.31b	75.00±4.42a
11	‘京华幻彩’	0b	2.08±2.95b	16.67±5.89a

品种 Variety	分化培养基编号 Differentiation medium number	细胞分裂素质量浓度 Cytokinin mass concentration/（mg·L^-1）		分生结节数量 Number of meristematic nodules	分化数量 Number of differentiation	分化率 Differentiation rate/%
品种 Variety	分化培养基编号 Differentiation medium number	CPPU	TDZ	分生结节数量 Number of meristematic nodules	分化数量 Number of differentiation	分化率 Differentiation rate/%
‘07-LB-19’	W1	0.5	0.5	15	2	13.33±9.42
	W2	1.0	0.5	15	5	33.33±9.42
	W3	2.0		15	0	0
	W4	4.0		15	0	0
	W5	6.0		15	0	0
	W6	8.0		15	0	0
	W7	10.0		15	0	0
‘Oukan’	W1	0.5	0.5	15	0	0
	W2	1.0	0.5	15	5	33.33±18.86
	W3	2.0		15	0	0
	W4	4.0		15	0	0
	W5	6.0		15	0	0
	W6	8.0		15	0	0
	W7	10.0		15	0	0
‘Alice Harding’	W1	0.5	0.5	15	1	6.67±9.42
‘Alice Harding’	W2	1.0	0.5	15	0	0

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