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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (4): 475-479.doi: 10.7525/j.issn.1673-5102.2006.04.006

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Cloning and sequence analysis of gene encoding plasma aquaporin of Tamarix albiflonum

DONG Yu-Zhi;YANG Chuan-Ping;ZHANG Dao-Yuan;WANG Yu-Cheng   

  1. 1.College of Forestry, Northeast Forestry University, Harbin 150040 2.College of Forestry, Xinjiang Agricultural University, Urumqi 8300523.Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi 830011
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-20 Published:2006-07-20
  • Contact: YANG Chuan-Ping
  • Supported by:

Abstract: Plant aquaporins are water-selected-channels in plants and are involved in seed germination, cell elongation, stoma movement, fertilization and so on. Some plant aquaporins also play an important role in response to drought stress. In this paper the gene encoding the Tamarix albiflonum Aquaporin (AQP) was amplified by 5′rapid amplification of cDNA end (RACE) based on the sequence information obtained from the expressed sequence tag of subtractive hybridization library constructed under PEG6000 stress. The cDNA of the Tamarix albiflonum AQP gene is 1043 bp long, encoding a protein of 287 amino acids with a predicted molecular mass of 30.9KDa,has 6 transmembrane regions, and possessing the MIP family signal consensus sequence SGXHXNPAVT and the higher plant PIP highly conservative sequence GGGANXXXXGY and TGI/TNPARSL/ FGAAI/VI/VF/YN. A comparative molecular analysis of nucleotide sequence in NCBI databases showed that it shared 95% homology with the gene of Arabidopsis thaliana (MIP-C), with a theoretical isoelectric point of 8.84.

Key words: Tamarix albiflonum, plant aquaporin, gene cloning, sequence analysis

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