Abstract: Primers were designed according to the cDNA sequence of tea CsiHPL1. RT-PCR method was used to clone CsiHPL1 from Longjing 43′. The expression of CsiHPL1 under biotic and abiotic stress and subcellular localization were analyzed. The results showed CsiHPL1 contains an open reading frame of 1 476 bp which encodes a protein of 491 amino acids. This gene was predicted as a 13-HPL gene. Tea geometrid feeding, wounding and JA treatment upregulated the expression levels of CsiHPL1. The total sequence of this gene was fused with GFP to construct a binary vector for tobacco transient transformation. Under confocal laser-scanning microscopy, green fluorescent signals were localized in chloroplasts in transgenic tobacco plants, suggesting that the gene encodes a protein targeting to chloroplast.

Key words: tea, hydroperoxide lyase gene, clone, expression