Observation on the Formation and Composition Analysis of Blue Pollen of Caryopterisincana

doi:10.7525/j.issn.1673-5102.2023.05.016

Abstract

Abstract:

In majority of plants， colorful pollen is rarely seen on the surface of pollen except light yellow. In this article， the existence of blue pollen was discovered in Caryopteris incana， and the color of anther and pollen at different developmental stages was observed by microscope， and the anthocyanin components of mature pollen were analyzed by UPLC/MS（Ultra Performance Liquid Chromatography-tandem mass spectrometry）. The results showed that mature anthers and pollens were blue due to the rich anthocyanins. The color of anther and pollen changed obviously at young buds， early flowering buds and flowering stages， which were light yellow， light blue and blue purple respectively. The blue pigments of pollen mainly existed in the surface of pollen coat and tapetal cells by the cross-section observation. The UPLC/MS analysis showed that there were six kinds of anthocyanins on the pollen surface at the flowering stage， among which the main blue components were Delphinidin 3-O-glucoside and Malvidin 3，5-O-diglucoside， followed by Peonidin， Cyanidin and Petunidin， while the content of Pelargonidin was the lowest.

Key words: Caryopteris incana, anther, pollen, anthocyanin

CLC Number:

Q949.777.6

Qinghua YANG, Binjie GE, Dasheng ZHANG. Observation on the Formation and Composition Analysis of Blue Pollen of Caryopterisincana[J]. Bulletin of Botanical Research, 2023, 43(5): 794-800.

Figures/Tables 4

Fig.1

Fig.2

Table 1

Fig.3

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序号 No.	成分类别 Composition	质量分数 Mass fraction/（μg·g^-1）
1	矢车菊素-3，5-O-二葡糖苷Cyanidin 3，5-O-diglucoside	0.098±0.009
2	矢车菊素-3-O-葡糖苷Cyanidin 3-O-glucoside	0.536±0.193
3	飞燕草素-3-O-半乳糖苷Delphinidin 3-O-galactoside	0.059±0.027
4	飞燕草素-3-O-葡糖苷Delphinidin 3-O-glucoside	1.720±0.260
5	锦葵色素-3，5-O-二葡糖苷Malvidin 3，5-diglucoside	1.071±0.381
6	锦葵色素-3-O-葡糖苷Malvidin 3-O-glucoside	0.010±0.009
7	天竺葵色素-3，5-O-二葡糖苷Pelargonidin 3，5-O-diglucoside	0.212±0.074
8	天竺葵色素-3-O-丙二酰葡糖苷Pelargonidin 3-O-（6-O-malonyl-β-D-glucoside）	0.044±0.009
9	天竺葵色素-3-O-葡糖苷Pelargonidin 3-O-glucoside	0.039±0.019
10	芍药花色素-3，5-O-二葡萄糖苷Peonidin 3，5-O-diglucoside	0.114±0.032
11	芍药花色素-3-O-葡糖苷Peonidin 3-O-glucoside	0.749±0.328
12	矮牵牛花色素-3，5-O-二葡糖苷Petunidin 3，5-O-diglucoside	0.055±0.028
13	矮牵牛花色素-3-O-丙二酰葡糖苷Petunidin 3-O-（6-O-malonyl-β-D-glucoside）	0.287±0.083
14	矮牵牛素-3-O-葡糖苷Petunidin 3-O-glucoside	0.119±0.035

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