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›› 2007, Vol. 27 ›› Issue (5): 593-595.doi: 10.7525/j.issn.1673-5102.2007.05.019

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The method for RNA isolating from leaves of Phellodendron amuranse

ZU Yuan-Gang;WANG Yan-Bing;WANG Hui-Mei;SUN Lian-Hui   

  1. Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin　150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-20 Published:2007-09-20
  • Contact: ZU Yuan-Gang

Abstract: A method of rapid isolation of total RNA from leaves of Phellodendron amuranse Rupr. was developed. RNA was extracted by guanidine hydrochloride, Trizol regent, and CTAB, respectively with analysis the rate of yield, purity and electrophoretograms. The A260/A280 of RNA extracted by guanidine hydrochloride was 1.928 with two clear bands of 28S and 18S and good integrity. The feather of RNA by this method was with less cost, fast, high purity and quantity, which can meet the demands of mRNA isolation, cDNA library construction and Northern blotting.

Key words: Phellodendron amuranse, leaves, RNA, isolation