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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (4): 461-464.doi: 10.7525/j.issn.1673-5102.2006.04.002

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Regeneration of transgenic maize plants with MDMV CP gene

ZHOU Xiao-Mei;JIA Wei-Long;ZHAO Yun-Yun   

  1. (1.School of Life Science and Technology, Shanxi University, Taiyuan 030006) (2.Department of Chemistry, Tianjin University, Tianjin 300072) (3.Department of Biology, Capital Normal University, Beijing 100037)
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-20 Published:2006-07-20
  • Contact: ZHAO Yun-Yun
  • Supported by:

Abstract: The maize calli derived from immature of inbred line Z31 were used for transformation mediated by microprojectile bombardment. The fertile transformants were obtained from resistant calli which had been subcultured on selecting medium containing 6 mg·L-1 Bialaphos for 3 months. Successful genetic transformation was confirmed by PCR,PCR-Southern blot assay and DNA dot hybridization. The incidence of virus disease was low on transgenic plants, thus showing that it had a certain resistance to MDMV.

Key words: Maize, CP gene, transgenic plant, microprojectile bombardment

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