Cloning and Expression Analysis of BRASSINAZOLE RESISTANTANT1 (BZR1) Gene from Fraxinus mandshurica

doi:10.7525/j.issn.1673-5102.2021.05.013

Abstract

Abstract:

To explore the expression characteristics of BZR1 gene under abiotic stress and hormone induction of brassinosteroid（BR） signaling on the growth and development of Fraxinus mandshurica and response to environmental stress，the target gene was cloned from F. mandshurica by PCR and its molecular structures were analyzed by bioinformatics. The expression patterns of FmBZR1 under low temperature， salt stress and ABA， IAA and GA₃ hormones were investigated by real-time fluorescence quantitative PCR. Bioinformatics analysis showed that the FmBZR1 gene was 984bp， encoding 327 amino acids. FmBZR1 was a hydrophilic protein， and had high homology with BZR1 protein of Olea europaea var. sylvestris. The results of abiotic stress and hormone induction showed that the expression of FmBZR1 gene was significantly different from that of control group. The highest expression level of FmBZR1 gene was at 6 h after low temperature treatment and 24 h after salt treatment， which were 1.98 times and 10.13 times higher than that of the control group. After stress with ABA， IAA and GA₃ for 3 h， the expression of FmBZR1 gene was the lowest， which was 0.52 times， 0.41 times and 0.50 times of the control group； after treatment GA₃ for 24 h， the expression level of FmBZR1 gene was the highest， which was 6.23 times of the control group. FmBZR1 gene played a key role in the development and stress process of F. mandshurica.

Key words: Fraxinus mandshurica, FmBZR1, gene cloning, stress treatment, expression analysis

CLC Number:

S792.41

Xin-Tong JI, Lei YU, Ya-Guang ZHAN. Cloning and Expression Analysis of BRASSINAZOLE RESISTANTANT1 (BZR1) Gene from Fraxinus mandshurica[J]. Bulletin of Botanical Research, 2021, 41(5): 744-752.

Figures/Tables 11

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引物名称 Primer name	引物序列（5′-3′） Primer sequence	用途 Usages
FmBZR1-F	5′-ATGTGGGAAGCTGGAGGATCA-3′	PCR扩增引物 Primers for PCR amplification primer
FmBZR1-R	5′-TTACATCCGGGTGTTTCCACTT-3′	PCR扩增引物 Primers for PCR amplification primer
qFmBZR1-F	5′-ATGCAGGGTTAAGGGCACAAGG-3′	荧光定量PCR引物 Primers for qRT-PCR
qFmBZR1-R	5′-ACCATCAGGCTCCACGATCCAA-3′	荧光定量PCR引物 Primers for qRT-PCR
Tu-F	5′-AGGACGCTGCCAACAACTTT-3′	荧光定量PCR内参基因引物 Primers for qRT-PCR internal reference gene
Tu-R	5′-TTGAGGGGAAGGGTAAATAGTG-3′	荧光定量PCR内参基因引物 Primers for qRT-PCR internal reference gene

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