Effects of Exogenous Salicylic Acid on Physiological and Electrical Impedance Parameters of Trollius chinensis Seedlings under High Temperature Stress

doi:10.7525/j.issn.1673-5102.2020.04.008

Abstract

Abstract: In order to solve the problem of poor growth of Trollius chinensis seedlings during summer in low altitude areas of North China, the effects of salicylic acid spraying on physiological and electrical impedance parameters of T.chinensis seedlings under high temperature stress were studied for improving the heat resistance of T.chinensis. With T.chinensis seedlings as experimental materials, exogenous salicylic acid(SA) was sprayed with four concentration gradients(0.5, 1.0, 1.5 and 2.0 mmol·L^-1) and placed in an artificial climate chamber for high temperature stress test at 38℃ on day and 30℃ at night. The results showed that spraying 0.5-1.0 mmol·L^-1 salicylic acid could effectively reduce the heat injury index, relative conductivity and malondialdehyde(MDA) content of T.chinensis seedlings, alleviating chlorophyll degradation, significantly increase soluble sugar content, free proline content and superoxide dismutase(SOD) activity, among which 1.0 mmol·L^-1 salicylic acid(SA) had the best treatment effect, indicating that spraying SA with appropriate concentration could improve the heat resistance of T.chinensis seedlings, so as to alleviate the high temperature damage. The correlation analysis of each index showed that the heat injury index was positively extremely correlated with relative conductivity, negatively significantly correlated with total chlorophyll content, soluble sugar content and extracellular resistivity(r_e)(P<0.05), positively significantly correlated with intracellular resistivity(r_i) and Pro content, and positively significantly correlated with relaxation time(τ) and Pro content and SOD activity. Therefore, the resistance parameters such as extracellular resistivity, intracellular resistivity and relaxation time that can be used as an evaluation index of response of T.chinensis to high temperature stress.

Key words: Trollius chinensis Bunge., salicylic acid, high temperature stress, physiological, electrical impedance

CLC Number:

S682.19

LIU Jin-Yu, GAO Yue-Hao, HUANG Jin-Shuo, ZHANG Qin. Effects of Exogenous Salicylic Acid on Physiological and Electrical Impedance Parameters of Trollius chinensis Seedlings under High Temperature Stress[J]. Bulletin of Botanical Research, 2020, 40(4): 543-551.

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