Zinc Finger Protein OsZFP6 Expression Features and Functions in Saline-alkali Stress Response

doi:10.7525/j.issn.1673-5102.2020.03.014

Abstract

Abstract: C2HC type zinc finger protein was screened, encoded 304 amino acids and named OsZFP6. The expression level of OsZFP6 was increased by abiotic stress, including alkali(NaHCO₃) and H₂O₂ treatment in two-week old rice leaves but decreased in roots. Thus, it was speculated that OsZFP6 was involved in the regulation corresponding reaction pathways of resistance. We fused a green fluorescent protein(GFP) with it and found that OsZFP6 was located in the nucleus. Moreover, rice overexpressed OsZFP6 were more tolerant to NaHCO₃ treatments. The overexpressed plants had strong tolerance, and were higher than WT in plant height and fresh weight, but the contents of MDA and H₂O₂ in vivo were lower than non-transgenic plants, which indicated that OsZFP6 could enhance the resistance of plants by scavenging peroxide in response to NaHCO₃ stress. Preliminarily, it was speculated that OsZFP6 gene participated in the regulation of resistance and it may play a vital role in transcription regulation.

Key words: zinc finger protein, abiotic stress, rice, basic salt

CLC Number:

S511.5

LIU Jia-Li, HE Ming-Liang, LIU Chen-Xi, LIAO Xu, LI Xiu-Feng, GUAN Qing-Jie. Zinc Finger Protein OsZFP6 Expression Features and Functions in Saline-alkali Stress Response[J]. Bulletin of Botanical Research, 2020, 40(3): 424-432.

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