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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (2): 211-215.doi: 10.7525/j.issn.1673-5102.2017.02.008

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Expression Analysis of Rev1 and Rev3 of Halostachys caspica under Salt Stress

DU Chi1,2, ZHANG Ji1,2, ZHANG Fu-Chun1,2   

  1. 1. College of Life Science and Technology, Xinjiang University, Urumqi 830046;
    2. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046
  • Received:2016-11-03 Online:2017-03-15 Published:2017-03-11
  • Supported by:
    Supported by Special Funds of Xinjiang Key Laboratory(2014KL001)

Abstract: According to the transcriptome of Halostachys caspica under salt stress, the real-time PCR primers designed about Rev1 and Rev3 were referred to the ESTs of transcriptome sequencing. We established a method for detecting of mRNA transcription of Revs by Real-time PCR, and the transcription level of Revs in different concentration of NaCl present. Two genes have similar expression pattern, the expression levels remain stable under 100 mmol·L-1 NaCl treatment. As the concentration of NaCl was increased and the stress time was prolonged, the genes relative expression levels were also gradually increased. HcRev1 gene in 700 mmol·L-1 NaCl stress for 14 d was rapidly up-regulated and reached the peak level, which was 4.63-fold of the control. HcRev3 gene in 300 mmol·L-1 NaCl stress for 14 d was 15.55-fold of the control with significant differences. Both HcRev1 and HcRev3 genes were induced by salt stress, although there were different expression levels of HcRev1 and HcRev3 genes, which were involved in the DNA damage repair during salt stress.Our study could be used to clarify the regulatory function of HcRev1 and HcRev3 genes between DNA damage-repair and salt resistance in plants.

Key words: Halostachys caspica, HcRev1 gene, HcRev3 gene, salt stress, expression analysis

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