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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (3): 318-322.doi: 10.7525/j.issn.1673-5102.2011.03.012

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Vector Construction of Shoot-specific Promoter Driven Prosystemin and the Acquisition of Transgenic Plants

ZHAO Xue-Qin;LIU Wei-Zhong;JIANG Hong-Ling;ZHANG Hai-Yan*   

  1. 1. College of Life Sciences,Shanxi Normal University,Linfen　041000；2.The Center for Genome Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing　100101；3.The Research Center for Molecular and Developmental Biology,Institute of Botany,Chinese Academy of Sciences,Beijing　100093
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
  • Contact: ZHANG Hai-Yan
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Abstract: The objective of this study was to clone a shoot-specific promoter, it is the promoter of cab2(chlorophyll a/b binding protein 2, cab2) gene. The promoter can drive the expression of prosystemin (PS) and GFP fusion vector, then get the transgenic plants. The plasmids were transformed into Arabidopsis by using Agrobacterium tumefaciens-mediated method. The semi-quantitative RT-PCR method and laser scanning confocal microscope (LSCM) were used to observe the expression and subcellular localization of promoter-driven PS-GFP. Using Arabidopsis genomic DNA as a template, the sequence of promoter cab2 was amplified by high fidelity PCR enzyme, then connected it with the PS and GFP fusion vector (SlPS). The LSCM observation showed that the gene driven by this promoter was normally expressed and located in the cytoplasm. The cab2 promoter was cloned, which can drive the expression and localization of the PS and GFP fusion protein.

Key words: Arabidopsis, cab2, shoot-specific promoter, prosystemin

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